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Supelco

apHera NH₂ (5 μm) HPLC Columns

L × I.D. 15 cm × 2 mm, HPLC Column

Sinônimo(s):

Amino Functionalized HPLC Column

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About This Item

Código UNSPSC:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

apHera NH2 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 2 mm

Materiais

stainless steel column

Agency

suitable for USP L82

linha de produto

apHera

embalagem

pkg of 1 ea

fabricante/nome comercial

apHera

Parâmetros

147 bar pressure (2133 psi)
4-50 °C temperature

técnica(s)

HPLC: suitable
LC/MS: suitable

C × D.I.

15 cm × 2 mm

matriz

polymeric

Grupo ativo da matriz

amino phase

tamanho de partícula

5 μm

tamanho de poro

300 Å

pH operacional

1-14

aplicação(ões)

food and beverages

técnica de separação

hydrophilic interaction (HILIC)
normal phase

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Descrição geral

apHera amino columns are based on covalently bonded polyamine specifically optimized for the separation of mono- and oligosaccharides. The elution order mono-, di-, tri-saccharide shows increased eluton volume with increased acetonitrile concentration and complete stability for both acidic and alkaline eluates. The small, robust PVA copolymer bead provides mechanical and chemical strength as well as high column efficiency. Conventional camion columns based on silica do not show long column life, perhaps due to hydrolysis of silica particle by the basic amino group. Since Supelco uses a strong alkaline compatible polymer, these problems are eliminated. Stable retention time and long column life are also characteristic of the column.

Features of Amino apHera Columns
  • High efficiency for carbohydrate analysis
  • Ideal for basic conditions/amphoteric detection
  • High selectivity mono- to oligosaccharides
  • pH range 2-13
  • 300 angstrom pore size
  • Stable, predictable retention
  • Long column life

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Informações legais

apHera is a trademark of Sigma-Aldrich Co. LLC

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Marine de Person et al.
Journal of chromatography. A, 1081(2), 174-181 (2005-07-26)
A new procedure based on hydrophilic interaction chromatography coupled to tandem mass spectrometry (ionisation process by pneumatically assisted electrospray in negative ion mode), is developed and validated for the simultaneous determination of underivatised taurine and methionine in beverages rich in
Khin Than Myint et al.
Analytical chemistry, 81(18), 7766-7772 (2009-08-26)
We have developed a practical method for the comprehensive analysis of polar anionic metabolites in biological samples with the use of a nano-LC/MS system. A polyamine-bonded polymer-based apHera NH2 column, which is compatible with ammonium carbonate buffer, effectively retained anionic

Protocolos

Protocol for LC/MS Analysis of Glyphosate and Metabolites on apHera™ NH2, 2 mm I.D. Column

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