The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.
53823-U
Ascentis® Express 90 Å C18 (2.7 μm) HPLC Columns
L × I.D. 10 cm × 2.1 mm, HPLC Column
Sinônimo(s):
Core-shell (SPP) Fused Core C18 HPLC column
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About This Item
Código UNSPSC:
41115700
eCl@ss:
32110501
NACRES:
SB.52
Preço e disponibilidade não estão disponíveis no momento.
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Nome do produto
Coluna de HPLC Ascentis® Express C18, 2,7 μm, 2.7 μm particle size, L × I.D. 10 cm × 2.1 mm
Materiais
stainless steel column
Nível de qualidade
Agency
suitable for USP L1
linha de produto
Ascentis®
Características
endcapped
fabricante/nome comercial
Ascentis®
embalagem
1 ea of
Parâmetros
60 °C temp. range
600 bar max. pressure (9000 psi)
técnica(s)
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
C × D.I.
10 cm × 2.1 mm
área da superfície
135 m2/g
Impurezas
<5 ppm metals
Matriz
Fused-Core particle platform
superficially porous particle
Grupo ativo da matriz
C18 (octadecyl) phase
tamanho de partícula
2.7 μm
tamanho de poro
90 Å
pH operacional
2-9
aplicação(ões)
food and beverages
técnica de separação
reversed phase
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Categorias relacionadas
Descrição geral
Colunas de HPLC Ascentis Express, através do uso tecnologia de partículas Fused-Core®, podem fornecer a alta velocidade e as altas taxas de eficiência de partículas com menos de 2 μm e, ao mesmo tempo, manter contrapressões menores. A combinação de alta eficiência e baixa contrapressão beneficia usuários de UPLC® (ou outro sistema de pressão ultra alta), além de usuários de HPLC convencionais.
Visite a página inicial do Ascentis Express para obter mais informações sobre essa nova tecnologia de colunas.
Visite a página inicial do Ascentis Express para obter mais informações sobre essa nova tecnologia de colunas.
Aplicação
Análise de HPLC de capsaicinas em pó de pimenta no Ascentis® Express C18, 2,7 μm
Análise de UHPLC/MS de óleo de milho no Ascentis® Express C30 em comparação com o Ascentis® Express C18
Análise de UHPLC de paracetamol e impurezas no Ascentis® Express C18, 2,7 μm
Análise de HPLC de parabenos no Ascentis®Express C18, 2,7 μm
Análise de UHPLC/MS de óleo de milho no Ascentis® Express C30 em comparação com o Ascentis® Express C18
Análise de UHPLC de paracetamol e impurezas no Ascentis® Express C18, 2,7 μm
Análise de HPLC de parabenos no Ascentis®Express C18, 2,7 μm
Informações legais
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
UPLC is a registered trademark of Waters
Aplicação
Nº do produto
Descrição
Preços
produto relacionado
Nº do produto
Descrição
Preços
pré-cartucho
Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Classificações de perigo
Skin Sens. 1
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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Chromatograms
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How can I measure my instrument bandwidth (IBW) and determine what Ascentis® Express HPLC Columns can be used with minimal efficiency loss created by too much internal instrument volume?
1 answer-
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Can Ascentis® Express HPLC Columns be used for LC-MS?
1 answer-
Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of Ascentis Express columns for high throughput UHPLC and LC-MS is that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.
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Can I use Ascentis Express on a UHPLC system?
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Yes. Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments. In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure. These benefits include the capability of providing fast HPLC and higher resolution chromatography. The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
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What is the carbon load for Ascentis® Express C18 HPLC Columns?
1 answer-
Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis® Express. Since Fused-Core™ particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles. The carbon coverage for Ascentis® Express C18 is 3.5 micromoles/meter-squared
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Is there anything special I need to do to my HPLC system to use Ascentis Express?
1 answer-
Nothing special is required to use Ascentis Express HPLC columns. To obtain the full benefits of Ascentis Express, one should minimize dispersion in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level. For more information, request Guidelines for Optimizing Systems for Ascentis Express Columns (T407102).
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Can I use Ascentis Express on any type of HPLC system?
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Ascentis Express HPLC columns are capable of use on standard HPLC systems as well as UHPLC systems. Columns are packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Do I need special fittings and tubing to connect Ascentis® Express HPLC Columns?
1 answer-
While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.
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What flow rate should I use with Ascentis® Express HPLC Columns?
1 answer-
Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize Ascentis Express column efficiency. The suggested starting point for flow rate for Ascentis Express columns: 1.6 mL/min for 4.6 mm ID; 0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.
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