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Discovery® C18 (5 µm) HPLC Columns

L × I.D. 25 cm × 4 mm, HPLC Column

Sinônimo(s):

C18 TM=["Discovery"] HPLC Column

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About This Item

Código UNSPSC:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Coluna de HPLC C18 Discovery®, 5 μm particle size, L × I.D. 25 cm × 4 mm

Materiais

stainless steel column

Agency

suitable for USP L1

linha de produto

Discovery®

Características

endcapped

fabricante/nome comercial

Discovery®

embalagem

1 ea of

Extensão da rotulagem

12% Carbon loading

Parâmetros

≤70 °C temp. range
400 bar pressure (5801 psi)

técnica(s)

HPLC: suitable
LC/MS: suitable

C × D.I.

25 cm × 4 mm

área da superfície

300 m2/g

cobertura de superfície

3 μmol/m2

Impurezas

<10 ppm metals

matriz

silica gel, high purity, spherical particle platform
fully porous particle

Grupo ativo da matriz

C18 (octadecyl) phase

tamanho de partícula

5 μm

tamanho de poro

180 Å

operating pH range

2-8

aplicação(ões)

food and beverages

técnica de separação

reversed phase

Procurando produtos similares? Visita Guia de comparação de produtos

Categorias relacionadas

Descrição geral

Use a coluna C18 Discovery® para qualquer método que especifique C18. A forma de pico, reprodutibilidade e estabilidade excepcionais significam que essa é a coluna ideal para todos os métodos de C18, desde os mais complicados até os de rotina.

Aplicação


  • Identification, isolation and characterization of dolutegravir forced degradation products and their cytotoxicity potential.: Utilizes the Discovery® C18 HPLC Column to study the stability and degradation of the antiretroviral drug dolutegravir, crucial for ensuring drug safety and efficacy (Saida et al., 2019).

  • Rapid, Refined, and Robust Method for Expression, Purification, and Characterization of Recombinant Human Amyloid beta 1-42.: This methodological study leverages the Discovery® C18 HPLC Column for the purification and analysis of amyloid beta peptides, contributing to research on Alzheimer′s disease (Prakash et al., 2019).

  • Development and validation of a HPLC electrochemical detection method to measure COMT activity as a tool in drug development.: Showcases the use of the Discovery® C18 HPLC Column in developing a novel analytical method for measuring catechol-O-methyltransferase (COMT) activity, instrumental in the development of psychiatric and neurological medications (Viljoen et al., 2019).

Características e benefícios

  • Excelente reprodutibilidade
  • Forma de pico excepcional para analitos básicos e ácidos
  • Separações por LC-MS estáveis, com pouco vazamento
  • Separação de peptídeos e proteínas pequenas
  • Menor hidrofobicidade que muitas colunas C18 comparáveis, o que propicia uma análise mais rápida

Produtos recomendados

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Informações legais

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de classe de armazenamento

13 - Non Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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T R Shantha Kumar et al.
Journal of pharmaceutical and biomedical analysis, 38(1), 173-179 (2005-05-24)
A simple, precise and accurate isocratic reverse-phase liquid chromatography method with programmed wavelength detection has been validated to quantify DRF-4367 and Phenol red, simultaneously for application in rat in situ single pass intestinal perfusion study to assess intestinal permeability of
Himal Paudel Chhetri et al.
Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society, 22(5), 483-487 (2014-12-05)
This study presents the optimization of a simple HPLC-UV method for the determination of metformin in human plasma. Ion pair separation followed by UV detection was performed on deproteinized human plasma samples. The separation was carried out on a Discovery
Ramisetti Nageswara Rao et al.
Journal of separation science, 32(9), 1312-1322 (2009-04-29)
Modafinil, adrafinil and their related substances were synthesized and analyzed by RP-LC with ESI-MS/MS. The ionization mode, polarity, cone voltage, and chromatographic conditions were evaluated. The optimum LC-MS conditions to obtain fragment ions indispensable for identification of the structures were
Abbas Khan et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(24), 2423-2429 (2011-07-26)
A novel isocratic reversed-phase high performance liquid-chromatography/ultraviolet detection method for simultaneous determination of cefdinir and cefixime in human plasma was developed and validated after optimization of various chromatographic conditions and other experimental parameters. Sample preparation based on a simple extraction
Sheelendra Pratap Singh et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(27), 2845-2851 (2011-09-06)
Tamoxifen is the agent of choice for the treatment of estrogen receptor-positive breast cancer. Tamoxifen is a substrate of P-glycoprotein (P-gp) and microsomal cytochrome P450 (CYP) 3A, and biochanin A (BCA) is an inhibitor of P-gp and CYP3A. Hence, it

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