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50139-U

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LiChrospher® RP-18 endcapped (5 µm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

Sinônimo(s):

TM=["LiChrospher"] C18 HPLC column

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About This Item

Código UNSPSC:
41115700
eCl@ss:
32110501
NACRES:
SB.52
Preço e disponibilidade não estão disponíveis no momento.

Nome do produto

LiChrospher® RP-18 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm, endcapped

Materiais

stainless steel column

Nível de qualidade

100

Agency

suitable for USP L1

linha de produto

LiChrospher®

Características

endcapped

fabricante/nome comercial

LiChrospher®

embalagem

1 ea of

Extensão da rotulagem

21% Carbon loading

Parâmetros

≤65 °C temperature
≤70 °C temp. range
400 bar pressure (5801 psi)

técnica(s)

HPLC: suitable

C × D.I.

25 cm × 4.6 mm

área da superfície

350 m2/g

Matriz

octadecyl
 silica particle platform
fully porous particle

Grupo ativo da matriz

C18 (octadecyl) phase

tamanho de partícula

5 μm

tamanho de poro

100 Å

pH operacional

2-7.5

aplicação(ões)

food and beverages

técnica de separação

reversed phase

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Descrição geral

LiChrosorb® RP-18 HPLC Column are classified as L1 USP columns and primarily used for reversed-phase HPLC of neutral, acidic and weak basic compounds. The totally porous irregular particles ensure reliable results.
LiChrospher® RP-18 HPLC column is an incredibly reliable and highly versatile, traditionally manufactured spherical silica-based support, designed for reversed-phase chromatography of acidic, neutral and weakly basic compounds - substances frequently found in all analytical fields. The optimal selectivity and performance of the column ensure that these parameters remain constant from batch to batch and from year to year.

Aplicação


  • HPLC-UV and GC-MS Methods for Determination of Chlorambucil and Valproic Acid in Plasma for Further Exploring a New Combined Therapy of Chronic Lymphocytic Leukemia.: This study illustrates the efficiency of the LiChrospher® RP-18 HPLC Column in a novel approach to analyzing the pharmacokinetics of chlorambucil and valproic acid, providing critical data for potential combined therapy options in treating chronic lymphocytic leukemia (Lipska et al., 2021).

  • Rapid Determination of Metronidazole and 2-Hydroxymetronidazole in Murine Blood Plasma.: Utilizes the LiChrospher® RP-18 HPLC Column to establish a rapid and accurate method for the analysis of metronidazole and its metabolites in blood plasma, enhancing the understanding of its pharmacological impacts and supporting more effective therapeutic strategies (Zemanova et al., 2022).

  • Application of chemometric approach for development and validation of high performance liquid chromatography method for estimation of ropinirole hydrochloride.: Demonstrates the application of the LiChrospher® RP-18 HPLC Column in optimizing a chemometrically enhanced method for the determination of ropinirole hydrochloride, aimed at improving treatment strategies for Parkinsons disease (Fatima et al., 2019).

  • Application of an HPLC-MS/MS method for Teicoplanin drug substance and related impurities, part 2: Identity assignment of related impurities.: This research highlights the capability of the LiChrospher® RP-18 HPLC Column in detailed impurity profiling of teicoplanin, critical for maintaining drug purity and efficacy in antibiotic treatments (Tengattini et al., 2019).

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Informações legais

LiChrosorb is a registered trademark of Merck KGaA, Darmstadt, Germany
LiChrospher is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

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Certificados de análise (COA)

Lot/Batch Number

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Questions

1–4 of 4 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. hello, is the column generation solution 0.1 ml of formic acid in 100 2-propanol?

    1 answer

    1. The column generation solution for this product is 0.1% formic acid in 99.9 mL of 2-propanol.
      Please view the link below: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/213/919/regeneracao-de-coluna.pdf

      Helpful?

  4. how to regenerate reversed phase column

    1 answer

    1. Please visit below link for the recommended column regeneration procedures: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/213/919/regeneracao-de-coluna.pdf

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