YEAST1

Yeast Transformation Kit

reagents for introducing plasmid DNA into yeast

• Sinônimo(s): lithium acetate yeast transformation
• Código UNSPSC: 12352200
• NACRES: NA.85

Propriedades

• grau: Molecular Biology
• Nível de qualidade: 200
• uso: kit sufficient for >100 standard transformations
• técnica(s): transformation: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

Sigma′s Yeast Transformation Kit contains all necessary reagents and controls for efficient transformation of yeast by the lithium acetate method.

Aplicação

Suitable for transformation of any strain of yeast.[1][2][3] Convenient, flexible and sensitive, positive transformants can be obtained with as little as 10 ng of DNA; the optimum efficiency is in the 0.1- 3 μg range.

Características e benefícios

• Easy and ready-to-use
• Requires as little as 10 ng of plasmid DNA
• Flexibility for any strain of yeast
• Sufficient for over 100 standard transformations

Componentes

The Yeast Transformation Kit contains:

• Transformation Buffer; 100 ml; 100 mM lithium acetate, 10 mM Tris HCl, pH 7.6, and 1 mM EDTA
• Plate Buffer; 100 ml; 40% PEG, 100 mM lithium acetate, 10 mM Tris HCl, pH 7.5, 1 mM EDTA
• Deoxyribonucleic acid from salmon teste, 10 mg/ml; 2 x 1 ml
• Control Yeast Plasmid DNA pRS316 carrying the ura gene; 10 μg
• Yeast Synthetic Drop-out Medium Supplement Without Uracil; 1 g

Princípio

Transformation with a plasmid complementing the mutated gene enables the transformant to grow on medium lacking the required component. Yeast cells are made competent for transformation by incubation in a buffered lithium acetate solution. Transformation is then carried out by incubating the cells together with transforming DNA and carrier DNA in a solution containing polyethylene glycol (PEG).

Componentes do kit também disponíveis separadamente

• D9156 Deoxyribonucleic acid, single stranded from salmon testes, For hybridization 2 x 1
• Y1501 Yeast Synthetic Drop-out Medium Supplements, without uracil 1 g

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable