Y2001

Yeast Synthetic Drop-out Medium Supplements

without histidine, leucine, tryptophan and uracil

• Sinônimo(s): Synthetic drop-out medium for yeast
• Número MDL: MFCD01095679
• Código UNSPSC: 12000000
• NACRES: ND.02

Propriedades

• Nível de qualidade: 100
• forma: powder
• aplicação(ões): food and beverages

Descrição

Aplicação

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

Componentes

The supplements contain all of the following components except as indicated:
Amino acids: All standard amino acids except for histidine, leucine, tryptophan and uracil. Each amino acid is present at a concentration of 76 mg/L.
Other nutrients: Adenine (18 mg/L), inositol (76 mg/L), p-aminobenzoic acid (8 mg/L)

Outras notas

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.

Quantidade

1.4 g will supplement 1 liter of medium.

Informações de segurança

• Código de classe de armazenamento: 13 - Non Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)