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T7009

Sigma-Aldrich

Trombina

lyophilized powder, ≥1,000 NIH units/mg protein (E1%/280, 18.3)

Sinônimo(s):

Fator IIa

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About This Item

Número CAS:
9002-04-4
Número da licença da enzima:
3.4.21.5 (BRENDA, IUBMB)
Número CE:
232-648-7
Número MDL:
MFCD00082072
Código UNSPSC:
12352204
NACRES:
NA.54
Preço e disponibilidade não estão disponíveis no momento.

Formulário

lyophilized powder

Nível de qualidade

200

atividade específica

≥1,000 NIH units/mg protein (E1%/280, 18.3)

peso molecular

37.4 kDa

Impurezas

HIV and HBsAg, source material tested negative

nº de adesão UniProt

P00734

aplicação(ões)

diagnostic assay manufacturing

temperatura de armazenamento

−20°C

Informações sobre genes

human ... F2(2147)

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Descrição geral

The F2 gene encodes thrombin protein and is mapped to human chromosome 11p11.2.[1]

Aplicação

Thrombin from human plasma has been:
  • dissolved in CaCl2 and added to poly ethylene glycolated fibrinogen/cell solution for fibrin production[2]
  • to polymerize fibrin on well plates[3]
  • used as an endogenous agonist proteinase for proteinase-activated receptors : PAR1, PAR3 and PAR4 for stimulation of cells[4]

Ações bioquímicas/fisiológicas

Protease serina que cliva seletivamente ligações Arg-Gly do fibrinogênio para formar fibrina e fibrinopeptídeos A e B.
Thrombin is an endolytic serine protease[5] which stimulates vascular damage induced-leukocytes, platelets, endothelial and mesenchymal cell production. This action is mediated via G-protein-coupled receptor.[6] It catalysis the cleavage of fibrinogen at Arg−Gly bonds to form fibrin and release fibrinopeptides A and B.[5] The predominant form of thrombin in vivo is the zymogen prothrombin (factor II), which is produced in the liver. Prothrombin is a coagulation activation factor, high levels of which indicates cancer.[5] Thrombin can be used to cleave fusion proteins. Cleavage of fusion proteins can be carried out at a thrombin:fusion protein ratio of 1:500.[7]

Definição da unidade

Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard

forma física

Lyophilized from saline sodium citrate buffer, pH 6.5

Nota de análise

The NIH assay procedure uses 0.2 ml diluted plasma (1:1 with saline) as a substrate and 0.1ml of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.

Outras notas

View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.

Exoneração de responsabilidade

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Inibidor

Nº do produto
Descrição
Preços

substrato

Nº do produto
Descrição
Preços

Pictogramas

Health hazard
GHS08

Palavra indicadora

Danger

Frases de perigo

H334

Declarações de precaução

P261 - P284 - P501

Classificações de perigo

Resp. Sens. 1

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Escolha uma das versões mais recentes:

Certificados de análise (COA)

Lot/Batch Number
0000410046
0000389789
0000371958
0000371961
0000410046

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Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Signal transduction for proteinase-activated receptor-2-triggered prostaglandin E2 formation in human lung epithelial cells
Kawao N, et al.
Journal of Pharmacology and Experimental Therapeutics, 315(2), 576-589 (2005)
B J Biemond et al.
Circulation, 96(5), 1612-1615 (1997-10-07)
In many case-control as well as epidemiological studies, increased lipoprotein(a) [Lp(a)] levels are considered to constitute an independent risk factor for premature coronary artery and cerebrovascular disease. Lp(a) resembles an LDL particle with an additional linked protein [apolipoprotein(a), apo(a)], whose
Capillary-like network formation by human amniotic fluid-derived stem cells within fibrin/poly (ethylene glycol) hydrogels
Benavides OM, et al.
Tissue Engineering: Part A, 21(7-8), 1185-1194 (2015)
New vectors for high level expression of recombinant proteins in bacteria
Hakes DJ and Dixon JE
Analytical biochemistry, 202(2), 293-298 (1992)
Protease-activated receptor 3 is a second thrombin receptor in humans
Ishihara H, et al.
Nature, 386(6624), 502-502 (1997)
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Artigos

Thrombin Factor IIa

Thrombin Factor IIa is an endolytic serine protease that selectively cleaves the Arg--Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.

Questions

1–8 of 8 Questions  

  1. How much is 100UN of thrombin(T6884)? what is the weight in mg?

    1 answer

    1. Both of these Thrombin products - T7009 and T6884 - are sold by activity or units. The amount of powder in the vial is lot specific and based on the activity reported in the Certificate of Analysis.

      T6884-100UN, lot SLCP0517 tested at 2736 uits per milligram. The 100UN vial contains 36ug of powder per vial. See the link below to review this Certificate of Analysis:
      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/T6884-100UNSLCP0517.pdf

      T7009-100UN, lot SLCN9908 tested at 1742 units per milligram. The 100UN vial contains 57ug of powder per vial. See the link below to review this Certificate of Analysis:
      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/T7009-250UNSLCN9908.pdf

      Helpful?

  2. How much does the T-7009-100UN weigh with and without packaging? Country of origin Germany?

    1 answer

    1. This product is packaged in units, the exact amount of product will depend on the lot-specific activity. For example, the SLCN9908 has a specific activity of 1742 units/ mg protein. The end user will receive 0.06 mg. The net weight of packaging is 1,000 mg. The product is manufactured in the USA.

      Helpful?

  3. What is the solution stability of Product T7009, Thrombin from human plasma?

    1 answer

    1. By analogy to thrombin from bovine plasma (T4648), we expect stock solutions of thrombin from human plasma to be stable for one week kept refrigerated at 2-8° C. Since thrombin solutions adsorb to glass, it is recommended to aliquot the solutions in plastic tubes and store frozen at -20°C or colder for long term storage.

      Helpful?

  4. What is the isoelectric point of Product T7009, Thrombin from human plasma?

    1 answer

    1. The isoelectric point (pI) range of the human thrombin isozymes is 6.35 - 7.6. The major isoform has a pI of 7.31.

      Helpful?

  5. For thrombin, how do NIH units compare to International Units (IU)?

    1 answer

    1. One NIH unit of thrombin is equivalent to approximately 1.8 International Unit (IU) of thrombin.

      Helpful?

  6. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  7. What is the solubility of Product T7009, Thrombin from human plasma?

    1 answer

    1. Thrombin from human plasma gave a clear very faint yellow solution when dissolved at 100 unit vial plus 1 ml of 28 mM sodium barbital, 0.125M sodium chloride, 1% BSA, 0.5% PEG, pH 7.35.  Thrombin from bovine plasma (T4648) is soluble in water at 10 mg/mL.

      Helpful?

  8. What is the cross reactivity of Thrombin?

    1 answer

    1. Thrombin from any vertebrate species will clot the fibrinogen of virtually any other species.  Fibrinogen from any mammalian source will cross-react with thrombin from any mammalian source, but it is not well known how thrombin and fibrinogen from different animals differ in their sequence.  When any mammalian thrombin is injected into a different mammal, clotting will occur.

      Helpful?

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