F4883

Fibrinogênio

35-65% protein (≥90% of protein is clottable).

• Sinônimo(s): Fator I
• Número CAS: 9001-32-5
• UNSPSC Code: 12352202
• EC Number: 232-598-6
• NACRES: NA.32
• MDL number: MFCD00131060
• Form: powder
• Biological source: human plasma
• Mol wt: α-chain 63.5 kDa, β-chain 56 kDa, γ chain 47 kDa (about 4% carbohydrate content), soluble dimer 340 kDa

Propriedades

• biological source: human plasma
• Quality Segment: 300
• description: essentially plasmin(ogen) free
• form: powder
• quality: 35-65% protein (≥90% of protein is clottable).
• mol wt: α-chain 63.5 kDa, β-chain 56 kDa, γ chain 47 kDa (about 4% carbohydrate content), soluble dimer 340 kDa
• concentration: 35-65% protein (biuret)
• technique(s): cell culture | mammalian: suitable
• solubility: 0.9% NaCl: soluble
• UniProt accession no.: P02671, P02675, P02679
• storage temp.: −20°C
• Gene Information: human ... FGA(2243), FGB(2244), FGG(2266)

Descrição

General description

Fibrinogen was the first blood coagulation factor to be identified, and it is a precursor of fibrin protein. This protein is composed of two chains, each of which is composed of three chains- α, β and γ. These three subunits are coded by three genes located in the human chromosomal locus 4q23-q32, and spans 51kb.[1]

Application

Fibrinogen from human plasma has been used for-

• fibrinogen adsorption assay performed on swCNTs (single-walled carbon nanotubes)
• the characterization of fibrinogen structure in the presence of CNT using CD (Circular dichroism) spectroscopy and temperature scan[2]
• the preparation of RBC suspension obtained from venous blood of antecubital vein, to assess RBC aggregability[3]
• fibrinolysis assay performed on mouse embryonic fibroblasts (MEFs)[4]
• the preparation of fibrinogen-labeled gold (Fgn/Au)[5]
• the quantification of in vitro angiogenesis by human microvascular endothelial cells[6] and
• the assessment of fibrin-induced apoptosis and differentiation-regulation in trophoblast obtained from term human placentas.[7]

Biochem/physiol Actions

O fibrinogênio é uma proteína de fase aguda que faz parte da cascata de coagulação de proteínas. O resultado final da cascata é a produção de trombina, que converte fibrinogênio em fibrina. A trombina proteolisa rapidamente o fibrinogênio, liberando o fibrinopeptídeo A. A perda desse pequeno peptídeo não é suficiente para tornar a molécula de fibrina resultante insolúvel, mas tende a formar complexos com as moléculas adjacentes de fibrina e fibrinogênio. Em seguida, a trombina cliva da fibrina um segundo peptídeo, o fibrinopeptídeo B, e, então, os monômeros de fibrina formados se polimerizam espontaneamente para formar um gel insolúvel. A fibrina polimerizada é mantida íntegra por forças não covalentes e eletrostáticas e estabilizada pela enzima de transamidação, fator XIIIa, que é produzida pela ação da trombina sobre o fator XIII. Os agregados insolúveis de fibrina (coágulos) e as plaquetas agregadas bloqueiam, então, o vaso sanguíneo danificado e impedem sangramento adicional. A quantidade de fibrinogênio no plasma pode funcionar como um indicador não específico da presença ou ausência de um processo inflamatório no organismo. O fibrinogênio originário de qualquer mamífero será clivado por uma trombina originária de qualquer mamífero.

Proteína de fase aguda que faz parte da cascata de coagulação de proteínas.

Fibrinogen is an acute-phase inflammatory protein, which plays an essential role in blood clotting. Coronary heart disease is linked to the plasma levels of fibrinogen in Chinese population, and might have potential as a marker and risk factor for the same.[1] A -455 allele of fibrinogen β (FGB) chain is linked with poor survival in Caucasian women with Finnish stroke.[8] Ischemic stroke preceded by hyperfibrinogenemia has poor outcome, which is independent of the baseline fibrinogen concentration.[9]

Features and Benefits

Source material has tested negative for HIV and HBsAg.

Physical form

Lyophilized powder containing sodium citrate and sodium chloride.

Analysis Note

Protein determined by biuret

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Informações de segurança

• Classe de armazenamento: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)