SRP3017

BMP-6 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

• Sinônimo(s): VGR
• Código UNSPSC: 12352202
• NACRES: NA.32

Propriedades

• fonte biológica: human
• recombinante: expressed in HEK 293 cells
• Ensaio: ≥95% (HPLC); ≥95% (SDS-PAGE)
• Formulário: lyophilized
• potência: 0.03-0.06 μg/mL ED₅₀
• peso molecular: 26.2 kDa
• embalagem: pkg of 10 μg
• técnica(s): cell culture | mammalian: suitable
• Impurezas: <0.1 EU/μg endotoxin, tested
• nº de adesão UniProt: P22004
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C
• Informações sobre genes: human ... BMP6(654)

Descrição

Descrição geral

BMP6 (bone morphogenetic protein 6) is one of the BMPs which are secreted signaling proteins belonging to the transforming growth factor-β (TGF-β) superfamily.[1] This gene is localized to human chromosome 6p24.1.[2]
Recombinant human BMP-6 is a 26.2kDa homodimeric glycoprotein consisting of two 117 amino acid subunits, which correspond to amino acid residues 397 to 513 of the full-length BMP-6 precursor.

Ações bioquímicas/fisiológicas

BMPs (bone morphogenetic proteins) were initially identified from demineralized bone extracts capable of stimulating endochondral osteogenesis at extraskeletal sites in vivo. BMP6 is thought to be involved in early embryogenesis. In hepatocellular carcinoma (HCC) the expression of this protein is suppressed due to hypermethylation, and this is linked with poor patient outcomes.[1] This protein is also down-regulated due to CpG island methylation in CRC (colorectal carcinoma), although it is not yet been found to be linked with tumor progression or prognosis. BMP6 is involved in chondrocyte differentiation, and in fetal kidney and urinary system development.[3] It is thought to be involved in ovulation, where it elevates the accumulation of neutrophils in ovulatory follicles and reduces the influence of protease inhibitors.[4]

Sequência

VSSASDYNSS ELKTACRKHE LYVSFQDLGW QDWIIAPKGY AANYCDGECS FPLNAHMNAT NHAIVQTLVH LMNPEYVPKP CCAPTKLNAI SVLYFDDNSN VILKKYRNMV VRACGCH

forma física

Lyophilized from 10 mM Tris, pH 9.0.

Reconstituição

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable