SRP0291
Cathepsin L Active human
recombinant, expressed in FreeStyle™ 293-F cells, ≥90% (SDS-PAGE)
Sinônimo(s):
CATL, Major excreted protein (MEP)
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10 μG
R$ 2.809,00
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10 μG
R$ 2.809,00
About This Item
Código UNSPSC:
12352204
NACRES:
NA.54
Produtos recomendados
fonte biológica
human
recombinante
expressed in FreeStyle™ 293-F cells
Ensaio
≥90% (SDS-PAGE)
Formulário
aqueous solution
atividade específica
≥3900 pmol/min-μg
peso molecular
36 kDa
técnica(s)
inhibition assay: suitable
adequação
suitable for molecular biology
nº de adesão NCBI
aplicação(ões)
life science and biopharma
Condições de expedição
dry ice
temperatura de armazenamento
−70°C
Informações sobre genes
human ... CTSL(1514)
Descrição geral
Research area: Cell Signaling
Cathepsin L is a papain-like cysteine protease and belongs to the Clan A, Family C1.[1] It is composed of L domain of α-helix and an R domain of β-sheet in the spatial structure.[2]
Human cathepsin L (GenBank Accession No. NM_001912), amino acids 18-333, with C-terminal HIS tag, MW = 36 kDa, expressed in FreeStyle 293-F cells.
Cathepsin L is a papain-like cysteine protease and belongs to the Clan A, Family C1.[1] It is composed of L domain of α-helix and an R domain of β-sheet in the spatial structure.[2]
Human cathepsin L (GenBank Accession No. NM_001912), amino acids 18-333, with C-terminal HIS tag, MW = 36 kDa, expressed in FreeStyle 293-F cells.
Aplicação
Active human cathepsin L is useful for the activation of DPP1 (Cathepsin C). Active human cathepsin L has been used:
- to determine that N-acyl and N-sulfonyloxazolidine-2,4-diones are pseudo-irreversible inhibitors of serine proteases[3]
- to investigate the role of cathepsin B and L activity in the serum during the human aging process. [4]
- in inhibitory activity assay[2]
- to study the role of CTSL in COVID-19 infection[5]
Ações bioquímicas/fisiológicas
Cathepsin L is a cysteine protease, involved primarily in protein breakdown in the lysosome.[1] Cathepsin L degrades nuclear transcription factors and may affect cell cycle regulation. Cathepsin L is also involved in the immune system, specifically in degrading the invariant chain during major histocompatibility complex (MHC) class II processing, a crucial step in antigen presentation. Its expression in the thymus has been demonstrated to be vital for the development of natural killer cells. Additionally, Cathepsin L contributes to recycling processes during axon outgrowth and synapse formation in the developing postnatal central nervous system. Proteases are associated with the development and progression of cancer. Therefore, cathepsin L may be a potential therapeutic target in cancer treatment.[6]
Definição da unidade
One unit is defined as the amount of enzyme that will cleave 1 pmol of substrate per min at 37°C
forma física
Formulated in 50 mM MES, 400 mM NaCl, pH 5.0, and 10% glycerol.
Nota de preparo
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Informações legais
FreeStyle is a trademark of Invitrogen Corp.
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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Jana Ilgová et al.
Molecular and biochemical parasitology, 235, 111248-111248 (2019-12-25)
The gills of the common carp, whose mucosal surface belongs to the key defence mechanisms of piscine immunity, can be infested with both the larval and adult stage of Eudiplozoon nipponicum (Monogenea). Although on their own, monogeneans do not considerably
Peter Mundel et al.
Kidney international, 77(7), 571-580 (2009-11-20)
Proteinuria is a major health-care problem that affects several hundred million people worldwide. Proteinuria is a cardinal sign and a prognostic marker of kidney disease, and also an independent risk factor for cardiovascular morbidity and mortality. Microalbuminuria is the earliest
Ricardo A Orbegozo-Medina et al.
PloS one, 14(2), e0211035-e0211035 (2019-02-02)
Recombinant proteins expressed in E. coli are frequently purified by immobilized metal affinity chromatography (IMAC). By means of this technique, tagged proteins containing a polyhistidine sequence can be obtained up to 95% pure in a single step, but some host
Prohormone Thiol Protease
Vivian Y.H. Hook
Handbook of Proteolytic Enzymes (2013)
Jacqueline M Lankelma et al.
Life sciences, 86(7-8), 225-233 (2009-12-05)
Cathepsin L, a cysteine protease, is considered to be a potential therapeutic target in cancer treatment. Proteases are involved in the development and progression of cancer. Inhibition of activity of specific proteases may slow down cancer progression. In this review
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