SRP0228

SMURF2 Active human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

• Sinônimo(s): E3 ubiquitin ligase SMURF2, MGC138150, SMAD specific E3 ubiquitin protein ligase 2
• Código UNSPSC: 12352200
• NACRES: NA.32

Propriedades

• fonte biológica: human
• recombinante: expressed in baculovirus infected insect cells
• Ensaio: ≥80% (SDS-PAGE)
• forma: aqueous solution
• atividade específica: ≥0.00049 pmol/min-μg
• peso molecular: 72 kDa
• embalagem: pkg of 20 μg
• condição de armazenamento: avoid repeated freeze/thaw cycles
• concentração: >0.02 mg/mL
• nº de adesão NCBI: NM_022739
• nº de adesão UniProt: Q9HAU4
• Condições de expedição: dry ice
• temperatura de armazenamento: −70°C
• Informações sobre genes: human ... SMURF2(64750)

Descrição

Descrição geral

Human SMURF 2 (GenBank Accession No. NM_022739), amino acids 130-end, with N-terminal DDDDK tag (FLAG), MW = 72 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Aplicação

Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Definição da unidade

One unit is defined as the amount of enzyme required to transfer 1 pmol of ubiquitin to substrate/min at 37°C.

forma física

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide, 20% glycerol and 3 mM DTT.

Nota de preparo

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.