SAE0044
Choline Oxidase from Arthrobacter sp.
recombinant, expressed in E. coli ≥12 units/mg protein
Sinônimo(s):
COD, COX, ChOx, Choline: oxygen 1-oxidoreductase
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About This Item
Produtos recomendados
fonte biológica
bacterial (arthrobacter sp.)
Nível de qualidade
recombinante
expressed in E. coli ≥12 units/mg protein
forma
powder
atividade específica
≥12 units/mg protein
técnica(s)
activity assay: suitable
nº de adesão UniProt
temperatura de armazenamento
−20°C
Descrição geral
Research area: Cell Signaling
Choline oxidase from Arthrobacter sp. is a flavoprotein, which is a member of the glucose–methanol–choline (GMC). The codA gene encodes the choline oxidase enzyme.
Choline oxidase from Arthrobacter sp. is a flavoprotein, which is a member of the glucose–methanol–choline (GMC). The codA gene encodes the choline oxidase enzyme.
Aplicação
Choline oxidase from Arthrobacter sp. has been used in the enzymatic determination of choline in milk using a flow injection analysis (FIA) system with potentiometric detection. It has been used for fluorescent detection of choline released during autotaxin (ATX) activity in conditioned media and plasma.
Ações bioquímicas/fisiológicas
Choline oxidase from Arthrobacter sp. is a flavoprotein, which is a member of the GMC-oxidoreductase family. The enzyme catalyzes the four-electron-oxidation of choline to glycine betaine via the intermediate betaine aldehyde, in two sequential FAD-dependent reaction steps. The enzyme is useful for enzymatic determination of phosphatidylcholine by coupling with phospholipase D or determination of sphingomyelin by coupling with sphingomyelinase and for cholinesterase activity assays.Choline oxidase (COD) mediating glycine betaine biosynthetic pathway plays a vital role in creating stress resistant transgenic plants.
Definição da unidade
One unit will form 1 μmole of H2O2 with oxidation of 1 μmole of choline to betaine aldehyde per min at pH 8.0 at 37 °C. Note: During the conversion of choline to betaine by choline oxidase, 2 μmoles of H2O2 are produced for every μmole of choline.
Nota de preparo
It is recommended to reconstitute choline oxidase in phosphate buffer at pH 7.5 supplemented with 10mM EDTA. Reconstituted material can be stored at 2-8 °C for at least 2 weeks.
Armazenamento e estabilidade
Tightly closed.Dry. Keep locked up or in an area accessible only to qualified or authorized
persons
persons
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Resp. Sens. 1
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
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The use of bacterial choline oxidase, a glycinebetaine-synthesizing enzyme, to create stress-resistant transgenic plants
Plant Physiology, 180-188 (2001)
Rapid, specific, and sensitive measurements of plasma sphingomyelin and phosphatidylcholine.
Hojjati M.R., and Jiang X.C.
Journal of Lipid Research, 47, 673-676 (2006)
Transformation of Arabidopsis thaliana with the codA gene for choline oxidase; accumulation of glycinebetaine and enhanced tolerance to salt and cold stress.
Hayashi H, et al.
The Plant Journal, 12(1), 133-142 (1997)
Transformation of Synechococcus with a gene for choline oxidase enhances tolerance to salt stress.
Deshnium P, et al.
Plant Molecular Biology, 29(5), 897-907 (1995)
New enzymatic assay of cholinesterase activity
Okabe, H. et al.,
Clinica Chimica Acta; International Journal of Clinical Chemistry, 80, 87-94 (1977)
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