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S7651

Sigma-Aldrich

Sphingomyelinase from Bacillus cereus

lyophilized powder, ≥100 units/mg protein

Sinônimo(s):

Sphingomyelin choline phosphohydrolase, Sphingomyelin phosphodiesterase

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About This Item

Número CAS:
9031-54-3
Número da licença da enzima:
3.1.4.12 (BRENDA, IUBMB)
Número CE:
232-869-9
Número MDL:
MFCD00132362
Código UNSPSC:
12352204
NACRES:
NA.54

R$ 750,00

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Formulário

lyophilized powder

Nível de qualidade

200

atividade específica

≥100 units/mg protein

peso molecular

36873 Da

composição

Protein, ~10% Lowry

temperatura de armazenamento

2-8°C

Informações sobre genes

Bacillus cereus ATCC 14579 ... BC0671(1203022)

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Descrição geral

Sphingomyelinase from Bacillus cereus (Bc-Smase) is a Mg2+-containing metalloenzyme.[1] Bacillus cereus sphingomyelinase induces hemolysis of sheep erythrocytes.[2]
Sphingomyelinase modulates adenosine triphosphate (ATP)-independent endocytosis of fibroblast and large vesicles of macrophages.[3] Sphingomyelinase causes hemolysis of human erythrocytes. It is also a potent virulence factor leading to invasive diseases.[4]

Aplicação

Sphingomyelinase from Bacillus cereus has been used:
  • in the measurement of membrane capacitance in brain slices[3]
  • for testing and confirming its silkworm-killing activity[4]
  • in enzyme activity assay and for elucidating its effect on hydrolysis of sphingomyelin in complex lipid mixtures[5]

Sphingomyelinase has been used in a study to investigate skeletal manifestations in pediatric and adult patients with Niemann Pick disease. [6]

Ações bioquímicas/fisiológicas

Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin.

Definição da unidade

One unit will hydrolyze 1.0 μmole of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C.

forma física

Lyophilized powder containing potassium phosphate buffer salts and stabilizer.

Pictogramas

Exclamation mark
GHS07

Palavra indicadora

Warning

Frases de perigo

H317

Classificações de perigo

Skin Sens. 1

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

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Certificados de análise (COA)

Lot/Batch Number
0000419321
0000419323
0000419323
0000419321
0000287995

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Masataka Oda et al.
Biochimica et biophysica acta, 1798(6), 1073-1080 (2010-03-11)
Bacillus cereus sphingomyelinase (Bc-SMase) induces hemolysis of sheep erythrocytes which contain large amounts of sphingomyelin. We investigated the mechanism of this hemolysis in comparison to that induced by Clostridium perfringens alpha-toxin. Pertussis toxin, a Gi-specific inhibitor, N-oleoylethernolamine, a ceramidase inhibitor
Lipid bilayers containing sphingomyelins and ceramides of varying N-acyl lengths: a glimpse into sphingolipid complexity
Jimenez-Rojo N, et al.
Biochimica et Biophysica Acta - Biomembranes, 1838(1), 456-464 (2014)
Melissa Wasserstein et al.
Journal of inherited metabolic disease, 36(1), 123-127 (2012-06-22)
Niemann-Pick disease (NPD) due to acid sphingomyelinase deficiency is a lipid storage disease resulting from the accumulation of sphingomyelin, predominantly within cells of the monocyte-macrophage system. In contrast to other lysosomal storage disorders, skeletal involvement in NPD has not been
Purification of a soil bacteria exotoxin using silkworm toxicity to measure specific activity
Usui K, et al.
Microbial Pathogenesis, 46(2), 59-62 (2009)
Masataka Oda
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan, 129(10), 1233-1238 (2009-10-03)
Bacillus cereus is one that causes of opportunistic human infections. Sphingomyelinase produced by B. cereus is assumed a virulence factor for the infection. Sphingomyelinase from Bacillus cereus (Bc-SMase) is Mg(2+)-containing metalloenzyme. Bc-SMase is a family of neutral SMase (nSMase) and
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Questions

  1. Hi for product number S7651, Sphingomyelinase from Bacillus cereus, once prepared how long can the Sphingomyelinase solution be kept and should it be kept at 2-8C or -20C?

    1 answer

    1. This product is stable for several days when stored refrigerated. Prepared solutions in aqueous buffers containing 50 % Glycerol are stable for extended periods when refrigerated.

      To review these product options please see the link below:
      https://www.sigmaaldrich.com/search/9031-54-3?focus=products&page=1&perpage=30&sort=relevance&term=9031-54-3&type=product

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