The protein content and enzymatic activity of this product vary from lot to lot. The protein content will range from 20% - 40%, and the activity will range from 10 units - 50 units per milligram of protein. As an example, a batch that reports a protein content of 20% and an activity of 10 units per milligram of protein will have a vial content of 12.5 mg for the 25 unit package size. The protein content and activity are reported in the product Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/p7633#product-documentation
P7633
Phospholipase C from Clostridium perfringens (C. welchii)
Type I, lyophilized powder, 10-50 units/mg protein
Sinônimo(s):
Lecithinase C, Lipophosphodiesterase I, PC-PLC, Phosphatidylcholine cholinephosphohydrolase
Faça loginpara ver os preços organizacionais e de contrato
Selecione um tamanho
25 UNITS
R$ 921,00
125 UNITS
R$ 1.228,00
500 UNITS
R$ 3.710,00
R$ 921,00
Check Cart for Availability
Selecione um tamanho
Alterar visualização
25 UNITS
R$ 921,00
125 UNITS
R$ 1.228,00
500 UNITS
R$ 3.710,00
About This Item
R$ 921,00
Check Cart for Availability
Produtos recomendados
fonte biológica
bacterial (Clostridium perfringens)
Nível de qualidade
tipo
Type I
Formulário
lyophilized powder
atividade específica
10-50 units/mg protein
composição
protein, 20-40%
características do produto alternativo mais ecológico
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
aplicação(ões)
diagnostic assay manufacturing
categoria alternativa mais ecológica
, Enabling
temperatura de armazenamento
−20°C
Informações sobre genes
Clostridium perfringens str. 13 ... plc(988262)
Procurando produtos similares? Visita Guia de comparação de produtos
Categorias relacionadas
Descrição geral
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in biodiesel research. For more information see the article in biofiles.
Aplicação
Phospholipase C (PLC) has been used to study adrenoceptor-mediated transmembrane signaling. It is also used to degrade inositol-containing phospholipids. Furthermore, it has been used to study 9E3 gene activation[1].
Ações bioquímicas/fisiológicas
Hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol; this enzyme will also hydrolyze the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids.
PLC hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol. This enzyme also hydrolyzes the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids. Phospholipase C is induced by thrombin and platelet-activating factor, forming 1,2-diacylglycerol and phosphatidic acid.
Definição da unidade
One unit will liberate 1.0 μmole of water soluble organic phosphorus from egg yolk L-α-phosphatidylcholine per min at pH 7.3 at 37 °C.
Inibidor
substrato
Nº do produto
Descrição
Preços
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Resp. Sens. 1
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
Escolha uma das versões mais recentes:
Certificados de análise (COA)
Lot/Batch Number
Não está vendo a versão correta?
Se precisar de uma versão específica, você pode procurar um certificado específico pelo número do lote ou da remessa.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Os clientes também visualizaram
R Spangler et al.
Proceedings of the National Academy of Sciences of the United States of America, 86(18), 7017-7021 (1989-09-01)
Induction of the transformation-related gene 9E3 by the v-src and v-fps gene products (v-Src and v-Fps) is blocked in chicken embryo fibroblasts depleted of protein kinase C (PKC). PKC agonists induce 9E3 gene expression. Protein kinase inhibitors block v-Src- and
Mojtaba Alimolaei et al.
Probiotics and antimicrobial proteins, 10(2), 251-257 (2017-04-13)
The alpha-toxin is one of the virulence factors of Clostridium perfringens for gas gangrene in humans and animals or necrotic enteritis in poultry. The C-terminal domain of this toxin ( cpa 247-370 ) was synthesized and cloned into pT1NX vector
Jennifer A Woyach et al.
Blood, 123(12), 1810-1817 (2014-01-15)
The Bruton's tyrosine kinase (BTK) inhibitor ibrutinib has outstanding activity in patients with chronic lymphocytic leukemia. Most patients experience lymphocytosis, representing lymphocyte egress from nodal compartments. This resolves within 8 months in the majority of patients, but a subgroup has
C Little et al.
Biochimica et biophysica acta, 391(2), 326-333 (1975-06-24)
1. The zinc content and metal ion dependence of phospholipase C(phosphatidylcholine cholinephosphohydrolase, EC 3.1.4.3) from Bacillus cereus have been examined. 2. The native enzyme contained about 2 atoms of tightly bound zinc/molecule. 3. Incubation of the enzyme with EDTA or
Sam Behjati et al.
Nature genetics, 46(4), 376-379 (2014-03-19)
Angiosarcoma is an aggressive malignancy that arises spontaneously or secondarily to ionizing radiation or chronic lymphoedema. Previous work has identified aberrant angiogenesis, including occasional somatic mutations in angiogenesis signaling genes, as a key driver of angiosarcoma. Here we employed whole-genome
-
what is the meaning of 10-50 units/mg protein.
1 answer-
Helpful?
-
-
P7633とP4039の精製方法の概要（方法名のみで差し支えございません）を開示して頂くことは可能でしょうか？
1 answer-
Unfortunately, the purification method for these products are considered proprietary. However, the following reference may be of interest:
Purification of Clostridium perfringens phospholipase C (alpha-toxin) by affinity chromatography on agarose-linked egg-yolk lipoprotein. T Takahashi et al. Biochimica et biophysica acta, 351(1), 155-171 (1974-05-10)
Helpful?
-
-
What are the differences in the purification methods between Products P7633 and P4039 Phospholipase C from Clostridium perfringens (C. welchii)?
1 answer-
The extraction and purification methods for our Phospholipase C products are proprietary, however higher unit per milligram values indicate a more purified product. Product P7633 contains a specific activity of 10-50 units/mg protein and the protein content is between 20.0 - 40.0 %. Product P4039 will contain a specific activity of ≥150 units/mg protein. The protein content for this product is between 5.0 - 30.0 %. The impact of the methods on the isoform profile of these products is not evaluated. The specific activity of each lot activity will be reported in the Certificate of Analysis. Please view the sample Certificate of Analysis:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/P7633-500UN0000218762.pdf
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/P4039-125UN0000185061.pdfHelpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
Are Products P7633 and P4039, Phospholipase C from Clostridium perfringens (C. welchii), prepared from the same strain?
1 answer-
Products P7633 and P4039, Phospholipase C, from Clostridium perfringens (C. welchii) are prepared from the same strain.
Helpful?
-
Active Filters
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica
