The integrity of Spermidine can be verified by Gas chromatography. The following publication may be of interest for protocol reference on Gas chromatography analysis of Spermidine.
1. J Mass Spectrom. 2009 Aug;44(8):1203-10. doi: 10.1002/jms.1597.
2. A quantitative GC-MS method for three major polyamines in postmortem brain cortex, PMID: 19514045
S0266
Spermidine
BioReagent, for molecular biology, suitable for cell culture, ≥98%
Sinônimo(s):
1,8-Diamino-4-azaoctane, N-(3-Aminopropyl)-1,4-diaminobutane
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1 G
R$ 424,00
5 G
R$ 1.270,00
25 G
R$ 5.069,00
R$ 424,00
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1 G
R$ 424,00
5 G
R$ 1.270,00
25 G
R$ 5.069,00
About This Item
Fórmula linear:
NH2(CH2)3NH(CH2)4NH2
Número CAS:
Peso molecular:
145.25
Beilstein:
1698591
Número CE:
Número MDL:
Código UNSPSC:
12352200
ID de substância PubChem:
NACRES:
NA.52
R$ 424,00
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Produtos recomendados
grau
for molecular biology
Nível de qualidade
linha de produto
BioReagent
Ensaio
≥98%
Formulário
solid or liquid
peso molecular
145.25 (AMU)
técnica(s)
cell culture | mammalian: suitable
índice de refração
n20/D 1.479 (lit.)
densidade
0.925 g/mL at 25 °C (lit.)
atividade externa
DNase, RNase, and protease, none detected
temperatura de armazenamento
room temp
cadeia de caracteres SMILES
NCCCCNCCCN
InChI
1S/C7H19N3/c8-4-1-2-6-10-7-3-5-9/h10H,1-9H2
chave InChI
ATHGHQPFGPMSJY-UHFFFAOYSA-N
Informações sobre genes
rat ... Ppm1a(24666)
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Descrição geral
Spermidine belongs to the natural polyamines, that are found in chloroplasts and in bacterial or eukaryotic cells.[1]
Aplicação
Ações bioquímicas/fisiológicas
Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; Stimulates T4 polynucleotide kinase activity.
Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; may be used for purification of DNA binding proteins. Stimulates T4 polynucleotide kinase activity.
Atenção
Solutions should be sterile-filtered, not autoclaved, if sterile solution is necessary. Spermidine deaminates with time; solutions should be stored frozen. Prepare new solutions frequently.
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Frases de perigo
Declarações de precaução
Classificações de perigo
Eye Dam. 1 - Skin Corr. 1B
Código de classe de armazenamento
8A - Combustible corrosive hazardous materials
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
233.6 °F - closed cup
Ponto de fulgor (°C)
112 °C - closed cup
Equipamento de proteção individual
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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Can the reconstituted S0266 solution, which has been sitting at room temperature for 3 months, still be used in CUT&Tag assays to stabilize nuclei? Also, how can the integrity of spermidine be tested? If there's no more spermidine available to test the solution, should more be purchased?
1 answer-
Helpful?
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What is the solvent that spermidine is dissolved in?
1 answer-
This product is a neat or pure liquid and has not been diluted in a solvent. However, to further dilute this product, it is recommended to use water at 50 mg/mL water.
Helpful?
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How to store S0266-5G? which comes as liquid and what is the molaritty of it? which solvent to use to dilute it?
1 answer-
This product can be stored in room temperature in its original format. The concentration of this product can be determined using the lot specific purity reported on the Certificate of Analysis, the density, and the compound molecular weight. This product has a density of 0.925 g/mL and a molecular weight of 145.25 g/mol. The minimum purity is 98%, so the molarity will vary slightly from lot to lot. The molarity can range from 6.2M (98%) to 6.4M (100%).
Molarity=Density x 1000 x Purity/MWThis product is supplied as a liquid or solid. A stock solution can be made by dissolving 1.45g in 10mL of water followed by sterile filtration with a 0.22um filter.
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How to store S0266-1G which comes as liquid? alliquoted and store at -20C? How long it can be stored at this condition?
1 answer-
Solutions should be stored as single-use aliquots at -20 ºC for no more than one month.
Helpful?
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How frequently should new solutions be made?
1 answer-
A stock solution can be made by dissolving 1.45g in 10mL of water followed by sterile filtration with a 0.22um filter. Solutions should be stored as single-use aliquots at -20 ºC for no more than one month.
Helpful?
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Is this a spermidine solution or a neat liquid, and what is the concentration?
1 answer-
Based on a density of 0.925 g/mL and a molecular weight of 145.25, this neat liquid has an effective molarity of 6.4 M.
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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