O meio RPMI 1640 foi desenvolvido no Instituto Roswell Park Memorial, em 1966, por Moore e seus colaboradores. Uma modificação do meio 5A de McCoy. Ele foi formulado para dar suporte a células linfoblastoides em cultura de suspensão, mas desde então demonstrou ser capaz de fornecer suporte a uma ampla gama de células dependentes de ancoragem. Destinado originalmente para ser usado com um suplemento de soro, demonstrou-se que o RPMI 1640 dá suporte a diversas linhagens celulares na ausência de soro. Ele também foi usado amplamente nos protocolos de fusão e no crescimento de células híbridas. Esse meio é adequado para a cultura de leucócitos humanos normais e neoplásicos.
Outras notas
Modified for autoclaving Without L-glutamine and sodium bicarbonate.
Quantidade
Formulated to contain 10.3 grams of powder per liter of medium.
Reconstituição
Supplement with 0.3 L-glutamine, 2.0 g/L sodium bicarbonate.
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Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
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