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R7876

Sigma-Aldrich

Ribonucleic acid, transfer from wheat germ

Type V, 15-19 units/mg solid

Sinônimo(s):

Transfer RNA, tRNA

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About This Item

Número CAS:
9014-25-9
Número MDL:
MFCD00132200
Código UNSPSC:
12352106
NACRES:
NA.55

grau

for molecular biology

Nível de qualidade

300

tipo

Type V

Formulário

lyophilized powder

atividade específica

15-19 units/mg solid

condição de armazenamento

desiccated

solubilidade

H2O: ~2 mg/mL, clear to hazy

temperatura de armazenamento

2-8°C

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Descrição geral

Wheat germ tRNA contains many different specific tRNA molecules.

Aplicação

Crude transfer RNA (tRNA), purified from wheat germ, contains many specific tRNA molecules that can be separated by different processes. Experiments for detailed investigation of structure and function using in vitro translation systems often require highly purified tRNA, which can be achieved by combinations of chromatographic separations and reversible biochemical modification of the tRNA. Wheat germ tRNA has been used for the purification of RNAse Z (tRNA 3′- processing endonucleases) from wheat germ. The purification included six steps with the most efficient one using an affinity column made of wheat germ tRNA, which the RNAse Z bound tightly.
Suitable for use as a carrier in nucleic acid purification and precipitation.

Componentes

tRNA is provided as a lyophilized powder that is soluble in water at a concentration of 2 mg/ml.

Definição da unidade

One unit will yield an absorbance of 1.0 at 260 nm in 1.0 mL of water (1 cm light path).

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

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Certificados de análise (COA)

Lot/Batch Number
0000397938
0000347113
0000397938
0000332134
0000294864

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