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P1851

Sigma-Aldrich

PIPES

BioPerformance Certified, suitable for cell culture

Sinônimo(s):

Piperazina-1,4-bis(ácido 2-etanossulfônico), Piperazina-N,N′-bis(ácido 2-etanossulfônico), Ácido 1,4-piperazinadietanossulfônico

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About This Item

Fórmula empírica (Notação de Hill):
C8H18N2O6S2
Número CAS:
Peso molecular:
302.37
Beilstein:
817713
Número CE:
Número MDL:
Código UNSPSC:
12161700
ID de substância PubChem:
NACRES:
NA.25

grau

BioPerformance Certified
for molecular biology

forma

crystalline powder

técnica(s)

cell culture | mammalian: suitable

Impurezas

endotoxin and Total Aerobic Microbial Count, tested

faixa de pH útil

6.1-7.5

pKa (25 °C)

6.8

pf

>300 °C (lit.)

solubilidade

1 M NaOH: 20 + 80 mL g, clear, colorless
soluble

aplicação(ões)

diagnostic assay manufacturing

atividade externa

DNAse, Exonuclease; NICKase, Endonuclease; RNAse; Protease, none detected

cadeia de caracteres SMILES

OS(=O)(=O)CCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O6S2/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16)

chave InChI

IHPYMWDTONKSCO-UHFFFAOYSA-N

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Aplicação


  • Sensitive Detection of Immunoglobulin G Stability Using in Real-Time Isothermal Differential Scanning Fluorimetry: Determinants of Protein Stability for Antibody-Based Therapeutics.: The use of PIPES buffer in this study was integral to maintaining the stability of immunoglobulin G during differential scanning fluorimetry. This method is crucial for evaluating the stability of antibody-based therapeutics (Moggridge et al., 2017).

Código de classe de armazenamento

13 - Non Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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An impurity, probably an anion, present in some batches of the buffer substances 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid (HEPES), 2-morpholinoethane sulfonic acid (Mes) and piperazine-1,4-bis(2-ethane sulfonic acid (Pipes), activates the soluble 5'-nucleotidase from rat kidney. The affinity of the enzyme for 5'-IMP
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