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OGS590

Sigma-Aldrich

PUC19 - HIGH COPY BLUE/WHITE CLONING PLASMID

plasmid vector for molecular cloning

Sinônimo(s):

cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.85
Preço e disponibilidade não estão disponíveis no momento.

Formulário

buffered aqueous solution

peso molecular

size 2686 bp

seleção de bactérias

ampicillin

Origem de replicação

pUC (500 copies)

clivagem do peptídeo

no cleavage

Promotor

Promoter name: Lac
Promoter activity: inducible
Promoter type: bacterial

gene repórter

none

Condições de expedição

ambient

temperatura de armazenamento

−20°C

Descrição geral

This high-copy number plasmid is the versatile industry standard cloning vector that can be used to insert and sub-clone genes using blue/white screening.

Aplicação

Cloning in a gene: PUC19 - HIGH COPY BLUE/WHITE CLONING PLASMID is a standard cloning plasmid used by thousands of laboratories for routine cloning. The plasmid can be used for blue-white screening and contains a diverse multiple cloning site that extends from the EcoRI to Hind3 restriction sites that allows DNA sequences to be inserted.

Sequência

To view sequence information for this product, please visit the product page

Nota de análise

To view the Certificate of Analysis for this product, please visit www.oxgene.com

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Código de classe de armazenamento

12 - Non Combustible Liquids

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

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Certificados de análise (COA)

Lot/Batch Number

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Alexander C Cerny et al.
PLoS genetics, 11(10), e1005578-e1005578 (2015-10-29)
Recycling of signaling proteins is a common phenomenon in diverse signaling pathways. In photoreceptors of Drosophila, light absorption by rhodopsin triggers a phospholipase Cβ-mediated opening of the ion channels transient receptor potential (TRP) and TRP-like (TRPL) and generates the visual
Diana Romero et al.
Carcinogenesis, 37(1), 18-29 (2015-10-28)
Dickkopf-3 (Dkk-3) is a secreted protein whose expression is downregulated in many types of cancer. Endogenous Dkk-3 is required for formation of acini in 3D cultures of prostate epithelial cells, where it inhibits transforming growth factor (TGF)-β/Smad signaling. Here, we
Geoffrey M Lynn et al.
Nature biotechnology, 33(11), 1201-1210 (2015-10-27)
The efficacy of vaccine adjuvants such as Toll-like receptor agonists (TLRa) can be improved through formulation and delivery approaches. Here, we attached small molecule TLR-7/8a to polymer scaffolds (polymer-TLR-7/8a) and evaluated how different physicochemical properties of the TLR-7/8a and polymer
Jin-Gyoung Jung et al.
PLoS genetics, 10(10), e1004751-e1004751 (2014-10-31)
The Notch3 signaling pathway is thought to play a critical role in cancer development, as evidenced by the Notch3 amplification and rearrangement observed in human cancers. However, the molecular mechanism by which Notch3 signaling contributes to tumorigenesis is largely unknown.

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