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BL21(DE3) Electrocompetent Cells

Escherichia coli, rod shaped

Sinônimo(s):

BL21 strain

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About This Item

Código UNSPSC:
41106202
NACRES:
NA.85

product name

BL21(DE3) Electrocompetent Cells, for protein expression

fonte biológica

Escherichia coli

grau

for molecular biology

modo de crescimento

adherent or suspension

morfologia

rod shaped

técnica(s)

microbiological culture: suitable

transformação celular

competent cell type: electrocompetent
transformation efficiency: ≥5 × 109 cfu/μg

Condições de expedição

dry ice

temperatura de armazenamento

−70°C

Categorias relacionadas

Descrição geral

The BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell.
Cloning efficiencies are increased 25-1,000 fold relative to other preparations of BL21 cells, which is essential for construction of complex expression libraries.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Características e benefícios

The unprecedented transformation efficiency of the BL21(DE3) Electrocompetent Cells (> 5 × 109 cfu/μg) eliminates the need for plasmid transfer from the cloning strain to the expression strain, saving days of work in a typical cloning and expression experiment

Componentes

  • BL21(DE3) electrocompetent cells
  • pUC 19 transformation control DNA
  • recovery medium for expression


Código de classe de armazenamento

10 - Combustible liquids

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Julianne M Troiano et al.
eLife, 10 (2021-01-16)
Under high light, oxygenic photosynthetic organisms avoid photodamage by thermally dissipating absorbed energy, which is called nonphotochemical quenching. In green algae, a chlorophyll and carotenoid-binding protein, light-harvesting complex stress-related (LHCSR3), detects excess energy via a pH drop and serves as
Felix Nicolaus et al.
eLife, 10 (2021-02-09)
We follow the cotranslational biosynthesis of three multispanning Escherichia coli inner membrane proteins in vivo using high-resolution force profile analysis. The force profiles show that the nascent chain is subjected to rapidly varying pulling forces during translation and reveal unexpected
Zev A Ripstein et al.
eLife, 9 (2020-01-10)
The ClpXP degradation machine consists of a hexameric AAA+ unfoldase (ClpX) and a pair of heptameric serine protease rings (ClpP) that unfold, translocate, and subsequently degrade client proteins. ClpXP is an important target for drug development against infectious diseases. Although
Salvatore Di Girolamo et al.
Microbial cell factories, 19(1), 170-170 (2020-08-29)
Miniaturization of biochemical reaction volumes within artificial microcompartments has been the key driver for directed evolution of several catalysts in the past two decades. Typically, single cells are co-compartmentalized within water-in-oil emulsion droplets with a fluorogenic substrate whose conversion allows
Brandon R Lowe et al.
eLife, 10 (2021-02-02)
Sequencing of cancer genomes has identified recurrent somatic mutations in histones, termed oncohistones, which are frequently poorly understood. Previously we showed that fission yeast expressing only the H3.3G34R mutant identified in aggressive pediatric glioma had reduced H3K36 trimethylation and acetylation
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Protocolos

BL21(DE3) Electrocompetent Cells

Efficient BL21(DE3) electrocompetent cell transformation protocol ensures optimal settings for high efficiency.

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