OGS588
PSF-CMV-BLAST - CMV PROMOTER BLASTICIDIN RESISTANT PLASMID
plasmid vector for molecular cloning
Sinônimo(s):
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
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About This Item
Código UNSPSC:
12352200
NACRES:
NA.85
Produtos recomendados
forma
buffered aqueous solution
peso molecular
size 4639 bp
seleção de bactérias
kanamycin
seleção de células de mamífero
blasticidin
Origem de replicação
pUC (500 copies)
clivagem do peptídeo
no cleavage
Promotor
Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian
gene repórter
none
Condições de expedição
ambient
temperatura de armazenamento
−20°C
Descrição geral
Here the CMV promoter regulates expression of blasticidin resistance allowing simple selection of transfected mammalian cells by blasticidin selection.
Promoter Expression Level: PSF-CMV-BLAST - CMV promoter blasticidin resistant plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
PSF-CMV-BLAST - CMV promoter blasticidin resistant plasmid is a blasticidin resistant plasmid where expression is controlled by the CMV promoter. It allows the production of mammalian cell lines carrying the plasmid.
Promoter Expression Level: PSF-CMV-BLAST - CMV promoter blasticidin resistant plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
PSF-CMV-BLAST - CMV promoter blasticidin resistant plasmid is a blasticidin resistant plasmid where expression is controlled by the CMV promoter. It allows the production of mammalian cell lines carrying the plasmid.
Aplicação
Cloning in a gene: PSF-CMV-BLAST - CMV promoter blasticidin resistant plasmid contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.
By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the molecular cloning vector.
Multiple cloning site notes: In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.
BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the molecular cloning vector.
Multiple cloning site notes: In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.
BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
Sequência
To view sequence information for this product, please visit the product page
Nota de análise
To view the Certificate of Analysis for this product, please visit www.oxgene.com
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Código de classe de armazenamento
12 - Non Combustible Liquids
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
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