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N3262

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NCTC 135 Medium

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

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About This Item

Código UNSPSC:
12171500
NACRES:
NA.75

forma

powder

técnica(s)

cell culture | mammalian: suitable

componentes

NaHCO3: no
L-glutamine: 0.13573 g/L
glucose: 1.0 g/L
phenol red: 0.02 g/L

Condições de expedição

ambient

temperatura de armazenamento

2-8°C

Categorias relacionadas

Descrição geral

NCTC 135 Medium is ideal for serum-free growth of mouse L-cells.

Aplicação

NCTC 135 Medium has been used:
  • to culture Philophthalmus sp. rediae in vitro
  • to culture Ehrlich ascites mouse tumor cell line
  • as a culture media for the differentiation of Rcho-1 cells

Quantidade

Formulated to contain 9.3 grams of powder per liter of medium.

Reconstituição

Supplement with 2.2 g/L sodium bicarbonate.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

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Bicarbonato de sódio powder, BioReagent, for molecular biology, suitable for cell culture, suitable for insect cell culture

Sigma-Aldrich

S5761

Bicarbonato de sódio

Meio RPMI-1640 With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

R8758

Meio RPMI-1640

Melanie M Lloyd et al.
Experimental parasitology, 129(2), 101-106 (2011-08-02)
The ability to culture parasites outside their host (i.e. in vitro) is essential for several aspects of parasitological research. Here, a culture medium for marine trematode parthenitae was optimized using Philophthalmus sp. rediae from the intermediate snail host, Zeacumantus subcarinatus.
Synthesis, characterization and antitumor activity of 1, 2-disubstituted ferrocenes and cyclodextrin inclusion complexes
Petrovski Z, et al.
Journal of Organometallic Chemistry, 693, 675-684 (2008)
Aiko Aoki et al.
The American journal of pathology, 188(11), 2474-2486 (2018-08-31)
Hypertensive disorder of pregnancy (HDP) is a serious pregnancy complication that is life threatening to both the mother and fetus. Understanding HDP pathophysiology is important for developing medical treatments. This study demonstrates the involvement of autophagy deficiency in adverse maternal
Melanie M Lloyd et al.
Experimental parasitology, 129(2), 101-106 (2011-08-02)
The ability to culture parasites outside their host (i.e. in vitro) is essential for several aspects of parasitological research. Here, a culture medium for marine trematode parthenitae was optimized using Philophthalmus sp. rediae from the intermediate snail host, Zeacumantus subcarinatus.
Aiko Aoki et al.
The American journal of pathology, 188(11), 2474-2486 (2018-08-31)
Hypertensive disorder of pregnancy (HDP) is a serious pregnancy complication that is life threatening to both the mother and fetus. Understanding HDP pathophysiology is important for developing medical treatments. This study demonstrates the involvement of autophagy deficiency in adverse maternal

Protocolos

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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