N2133

Neuraminidase from Clostridium perfringens (C. welchii)

Type X, lyophilized powder, ≥50 units/mg protein (using 4MU-NANA)

• Sinônimo(s): Acyl-neuraminyl Hydrolase, Receptor-destroying enzyme, Sialidase
• Número CAS: 9001-67-6
• Número da licença da enzima: 3.2.1.18 (BRENDA, IUBMB)
• Número CE: 232-624-6
• Número MDL: MFCD00131711
• Código UNSPSC: 12352204
• NACRES: NA.54

Propriedades

• tipo: Type X
• Nível de qualidade: 200
• Formulário: lyophilized powder
• atividade específica: ≥50 units/mg protein (using 4MU-NANA)
• temperatura de armazenamento: −20°C
• Informações sobre genes: Clostridium perfringens str. 13 ... nanI(988807)

Descrição

Descrição geral

Neuraminidase enzymes are glycoside hydrolase enzymes that catalyze hydrolysis of terminal sialic acid residues. The most well-known are the viral nearamidases, which promote influenza virus release. [1]

Aplicação

Neuraminidase from Clostridium perfringens has been used in a study to assess purification via affinity chromatography. [2] It has also been used in a study to investigate site-directed mutations of amino acids of the neuraminidase gene, nanH. [3]

Ações bioquímicas/fisiológicas

Neuraminidase can block attachment of type 3 reovirus to cell membranes. This effect is related to the ability of neuraminidase to hydrolysis sialic acid residues within cell surface receptors. [4]

Neuraminidase cleavage of sialic acid groups has been used to study recognition by antibodies of glycoprotein structures. The use of neuraminidase in the estimation of N-acetylneuraminic acid was compared favorably to two other methods.

Neuraminidases are used to cleave terminal N-acetyl neuraminic acid (sialic acid) from a variety of glycoproteins. The enzyme from Clostridium perfringens cleaves terminal sialic acid residues which are α-2,3- α-2,6- or α-2,8-linked to Gal, GlcNac, GalNAc, AcNeu, GlcNeu, oligosaccharides, glycolipids or glycoproteins. The relative rate of cleavage decreases in the order: α-2-3 > α-2-6 . α-2-8. Neuraminidase from C. perfringens cleaves α-2-3 linked sialic acid residues most efficiently, compared to A. ureafaciens, (Sigma N3642) which preferentially cleaves α-2-6 linked residues.

The use of neuraminidase to remove sialic acid residues from glycoproteins on cell surfaces has been frequently reported. Generally, procedures have indicated using neuraminidase in PBS at 37°C for 30 minutes, followed by several washings with PBS. Treatment of tissue sections with neuraminidase at much lower concentrations require longer incubation: for 1-4 U/mL in 0.1 M acetate buffer pH 4.2-5, from 2 to 20 hours at 37 °C.

Definição da unidade

One unit will hydrolyze 1.0 micromole of 2′-(4-Methylumbelliferyl)-alpha-D-N-actetylneuraminic acid per min at pH 5.0 at 37 °C.

Nota de preparo

Purified by affinity chromatography from Type VIII (N 5631)

Nota de análise

Package sizes based on 4MU-NANA units

Package sizes based on the 4MU-NANA units

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Danger
• Frases de perigo: H334
• Declarações de precaução: P261 - P284 - P501
• Classificações de perigo: Resp. Sens. 1
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)