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MAK186

Sigma-Aldrich

Xanthine/Hypoxanthine Assay Kit

Sufficient for 100 Colorimetric or Fluorometric tests

Sinônimo(s):

Xanthine and Hypoxanthine Detection Kit

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About This Item

Número CE:
Código UNSPSC:
12161503
NACRES:
NA.84

método de detecção

colorimetric
fluorometric

doença(s) relevante(s)

neurological disorders; rheumatological diseases

temperatura de armazenamento

−20°C

Descrição geral

Xanthine is a purine base present in body fluids, tissues, and plants cells. It is also found in urinary calculi. Xanthine is formed by the oxidation of hypoxanthine during the degradation of adenosine monophosphate (AMP) to uric acid. Xanthine/ hypoxanthine measurements in plasma and urine have been used to monitor allopurinol therapy. Cerebrospinal fluid (CSF) xanthine/hypoxanthine levels have been used as therapeutic guides and disease progression markers in hydrocephalus.

The Xanthine/Hypoxanthine Assay Kit is a highly sensitive assay for determining xanthine/hypoxanthine levels (ranging from 4-20 nmole/well for the colorimetric assay and 40-200 pmole/well for the fluorometric assay) in a variety of biological samples. Xanthine/hypoxanthine concentration is determined by an enzymatic assay, which results in a colorimetric (570 nm) or fluorometric (λex = 535/λem = 587 nm) product, proportional to the xanthine/hypoxanthine present.
Note: The fluorometric assay is ~10 times more sensitive than the colorimetric assay.

Características e benefícios

Compatible with high-throughput liquid handling systems.

Adequação

Suitable for the detection of Xanthine/Hypoxanthine in a variety of samples such as tissue, cells, and body fluids.

Princípio

The Xanthine/Hypoxanthine Assay Kit is a highly sensitive assay for determining xanthine/hypoxanthine levels (ranging from 4-20 nmole/well for the colorimetric assay and 40-200 pmole/well for the fluorometric assay) in a variety of biological samples. Xanthine/hypoxanthine concentration is determined by an enzymatic assay, which results in a colorimetric (570 nm) or fluorometric (λex = 535/λem = 587 nm) product, proportional to the xanthine/hypoxanthine present.

Outras notas

The fluorometric assay is ~10 times more sensitive than the colorimetric assay.

Somente componentes do kit

Nº do produto
Descrição

  • Xanthine Assay Buffer

  • Peroxidase Substrate

  • Xanthine Enzyme Mix

  • Developer

  • Xanthine Standard

Pictogramas

Health hazard

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Aquatic Chronic 3 - Resp. Sens. 1 - Skin Sens. 1

Código de classe de armazenamento

10 - Combustible liquids


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Konstantinos Feidantsis et al.
Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology, 190(4), 403-418 (2020-04-27)
For a comprehensive understanding of fish responses to increasing thermal stress in marine environments, we investigated tissue energetics, antioxidant levels, inflammatory and cell death responses in Sparus aurata (gilthead seabream) red muscle during exposure to elevated temperatures (24 °C, 26 °C, 30 °C)

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