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M7279

Sigma-Aldrich

N,N′-Methylenebisacrylamide

powder, for molecular biology, suitable for electrophoresis, ≥99.5%

Sinônimo(s):

BIS

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About This Item

Fórmula linear:
(CH2=CHCONH)2CH2
Número CAS:
Peso molecular:
154.17
Beilstein:
1706297
Número CE:
Número MDL:
Código UNSPSC:
41105319
ID de substância PubChem:
NACRES:
NB.22

grau

for molecular biology

Ensaio

≥99.5%

forma

powder

técnica(s)

electrophoresis: suitable

Impurezas

≤0.05% Free carboxylic acid (T)
≤50 mg/kg Total sulfur (as SO4 ) (ICP)

condutividade

≤10 μmho (2% solution)

traços de ânion

chloride (Cl-): ≤50 mg/kg

traços de cátion

Ca: ≤10 ppm
Cd: ≤5 ppm
Co: ≤5 ppm
Cr: ≤5 ppm
Cu: ≤5 ppm
Fe: ≤5 ppm
K: ≤50 ppm
Mg: ≤5 ppm
Mn: ≤5 ppm
Na: ≤50 ppm
Ni: ≤5 ppm
Pb: ≤5 ppm
Zn: ≤5 ppm

absorção

≤0.2 at 290 at 1%

atividade externa

DNase and RNase, none detected

cadeia de caracteres SMILES

C=CC(=O)NCNC(=O)C=C

InChI

1S/C7H10N2O2/c1-3-6(10)8-5-9-7(11)4-2/h3-4H,1-2,5H2,(H,8,10)(H,9,11)

chave InChI

ZIUHHBKFKCYYJD-UHFFFAOYSA-N

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Descrição geral

N,N′-Methylenebisacrylamide is commonly called as bis. It is used as a cross-linking agent in the preparation of polyacrylamide gels consisting of chains of acrylamide monomers. The concentration of N,N′-methylenebisacrylamide and acrylamide determines the pore size of the gel. The higher concentration of bis forms rigid gels with smaller pores due to more cross-linking leading to polymerization and vice versa.

Aplicação

N,N′-Methylenebisacrylamide has been used:
  • as a crosslinker in the monomer solution to prepare N,N-dimethyl acrylamide (DMAAm)-based cryogel and acrylamide (AAm)-based cryogel matrix
  • as a crosslinker to prepare tough hydrogel network
  • in the preparation of acrylamide-bisacrylamide stock solution for the preparation of polyacrylamide gel

To be mixed with acrylamide for making polyacrylamide gels for use in protein and nucleic acid electrophoresis.

Pictogramas

Skull and crossbonesHealth hazard

Palavra indicadora

Danger

Classificações de perigo

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Carc. 1B - Muta. 1B - Repr. 2 - STOT RE 1 Oral

Órgãos-alvo

Peripheral nervous system

Código de classe de armazenamento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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In cell affinity chromatography, type-specific cell separation is based on the interaction between cell-surface receptors and an immobilized ligand on a stationary matrix. This protocol describes the preparation of monolithic polyacrylamide and polydimethylacrylamide cryogel affinity matrices that can be used

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