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M0894

Sigma-Aldrich

Meio mínimo essencial de Eagle

Alpha Modification, with L-glutamine and sodium pyruvate, without ribonucleosides, deoxyribonucleosides and sodium bicarbonate, powder, suitable for cell culture

Sinônimo(s):

MEM

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.71

Nível de qualidade

500

forma

powder

técnica(s)

cell culture | mammalian: suitable

componentes

stable glutamine: no
Hanks’ salts (2% CO2): no
sodium pyruvate: yes
NaHCO3: no
phenol red: yes
glucose: yes
L-glutamine: yes
Earle’s salts (5% CO2): yes
HEPES: no

Condições de expedição

ambient

temperatura de armazenamento

2-8°C

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Categorias relacionadas

Descrição geral

This is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins.

Quantidade

Formulated to contain 10.1 grams of powder per liter of medium.

Reconstituição

Supplement with 2.2 g/L sodium bicarbonate.

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suplemento

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Pictogramas

Exclamation mark
GHS07

Palavra indicadora

Warning

Frases de perigo

H317

Classificações de perigo

Skin Sens. 1

Código de classe de armazenamento

13 - Non Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Alexander J Nelson et al.
Journal of lipid research, 61(2), 143-158 (2019-12-11)
Phospholipases A2 (PLA2s) catalyze hydrolysis of the sn-2 substituent from glycerophospholipids to yield a free fatty acid (i.e., arachidonic acid), which can be metabolized to pro- or anti-inflammatory eicosanoids. Macrophages modulate inflammatory responses and are affected by Ca2+-independent phospholipase A2
Jason W Ashley et al.
The Journal of biological chemistry, 291(44), 23268-23281 (2016-10-30)
Macrophages are important in innate and adaptive immunity. Macrophage participation in inflammation or tissue repair is directed by various extracellular signals and mediated by multiple intracellular pathways. Activation of group VIA phospholipase A2 (iPLA2β) causes accumulation of arachidonic acid, lysophospholipids
Tatsuki Kawasaki et al.
Development, growth & differentiation, 58(6), 530-545 (2016-06-01)
The periodontal ligament (PDL) is a connective tissue that attaches the tooth cementum to the alveolar bone and is derived from dental follicle cells (DFCs). The DFCs form fibroblasts, osteoblasts, cementoblasts, and PDL stem cells (PDLSCs). We previously reported homeobox
Shi-Yuan Sheu et al.
Cell transplantation, 29, 963689720927398-963689720927398 (2020-07-11)
Osteoporotic fracture is the main complication of osteoporosis (OP) and accounts for millions of injuries annually. Local intervention by intra-marrow injection has been a good option for preventing osteoporotic bone loss when the osteoporotic femoral fracture has been treated. In
Kateřina Valentová et al.
International journal of molecular sciences, 19(8) (2018-08-12)
Silymarin, an extract from milk thistle (Silybum marianum) fruits, is consumed in various food supplements. The metabolism of silymarin flavonolignans in mammals is complex, the exact structure of their metabolites still remains partly unclear and standards are not commercially available.
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Artigos

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

Protocolos

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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