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M3024

Sigma-Aldrich

Meio mínimo essencial de Eagle

with Earle′s salts and non-essential amino acids, without L-glutamine, phenol red and sodium bicarbonate, Modified, powder, suitable for cell culture

Sinônimo(s):

MEM

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.75

Nível de qualidade

500

forma

powder

técnica(s)

cell culture | mammalian: suitable

componentes

Earle’s salts (5% CO2): yes
L-glutamine: no
HEPES: no
NaHCO3: no
sodium pyruvate: no
phenol red: no

Condições de expedição

ambient

temperatura de armazenamento

2-8°C

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Descrição geral

Minimum Essential Medium Eagle contains 19 amino acids. The essential amino acids and the non-essential amino acids: L-ala, L-asn, L-asp, L-glu, L-gly, L-pro, and L-ser. This medium is preferred for the growth of adherent cells in the presence of reduced serum or as a base for the development of a serum-free medium. It is suitable for a wide variety of cells grown in monolayers.

Aplicação

Minimum Essential Medium Eagle has been used:

  • to culture endometrial adenocarcinoma Ishikawa cells for bisphenol studies
  • to culture MCF-7 cells for experimental treatments with hormone
  • to maintain zebrafish pineal glands for action spectrum analysis

Características e benefícios

Contains high concentrations of amino acids so the medium resembles the protein composition of cultured mammalian cells

Quantidade

Formulated to contain 9.3 grams of powder per liter of medium.

Reconstituição

Supplement with 0.292 g/L L-glutamine, 2.2 g/L sodium bicarbonate.

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suplemento

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Código de classe de armazenamento

13 - Non Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Artigos

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

Protocolos

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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