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K4002
Krebs-Ringer Bicarbonate Buffer
With 1800 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture
Sinônimo(s):
Bicarbonate Buffer Solution, Krebs Buffer, Krebs-Ringer Solution
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10 X 1 L
R$ 1.152,00
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10 X 1 L
R$ 1.152,00
About This Item
Código UNSPSC:
12161702
NACRES:
NA.75
Produtos recomendados
Nível de qualidade
Formulário
powder
técnica(s)
cell culture | mammalian: suitable
pH
6.1-6.7
solubilidade
water: 9.5 g/L, clear
temperatura de armazenamento
2-8°C
Descrição geral
Krebs-Ringer Bicarbonate Buffer acts as a salt solution and plays an essential role in tissue culture. A salt solution provides the cells with water and inorganic ions. It also helps in maintaining the osmotic balance and ideal pH in the medium. This bicarbonate buffered isotonic saline contains magnesium, potassium, sodium, and phosphate ions.
Aplicação
Quantidade
Formulated to contain 9.5 grams of powder per liter of medium.
Reconstituição
Supplement with 1.26 g/L sodium bicarbonate.
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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Protocolos
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
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What is the Department of Transportation shipping information for this product?
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How should Product K4002, Krebs-Ringer Bicarbonate Buffer, be dissolved?
1 answer-
Product No. K4002 is listed in a 10X1L package size, meaning that each of the ten containers has enough powder to make 1 Liter of KRB buffer. It can be dissolved in 1 Liter of either water or medium.
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What is the formula of Product K4002, Krebs-Ringer Bicarbonate Buffer (KRB)?
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The formulation for KRB can be found on the data sheet.
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Product K4002 - Krebs-Ringer Bicarbonate Buffer, is without calcium chloride and sodium bicarbonate. In the reconstitution steps, you advice to add 1.26g sodium bicarbonate. Should we also add some calcium chloride? in which amount?
1 answer-
Product K4002 - Krebs-Ringer Bicarbonate Buffer does not contain calcium. The addition of calcium is not required for osmolarity. If you need to have calcium, you can add calcium chloride between 1.25 to 2.5 mM. You may want to check the osmolarity and make sure it is not too high.Kreb Henseleit Buffer has 1.25 mM calcium chlorideKreb's Buffer has 2.5 mM calcium chlorideKreb' Ringer Superfusion solution contains 2.0 mM calcium chloride.Please see the attached document for different Krebs' buffer recipes.
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