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S8761

Sigma-Aldrich

Sodium Bicarbonate

Solution (7.5%), sterile-filtered, suitable for cell culture

Sinônimo(s):

aqueous sodium bicarbonate

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R$ 92,00
500 ML
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About This Item

Número CAS:
144-55-8
Número MDL:
MFCD00003528
Código UNSPSC:
12161702
ID de substância PubChem:
24899815
NACRES:
NA.75

R$ 92,00

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Nome do produto

Sodium bicarbonate solution, solution (7.5%), sterile-filtered, BioReagent, suitable for cell culture

esterilidade

sterile-filtered

linha de produto

BioReagent

Formulário

solution (7.5%)

técnica(s)

cell culture | mammalian: suitable

Impurezas

endotoxin, tested

pH

7.8-8.2

temperatura de armazenamento

2-8°C

cadeia de caracteres SMILES

[Na+].OC([O-])=O

InChI

1S/CH2O3.Na/c2-1(3)4;/h(H2,2,3,4);/q;+1/p-1

chave InChI

UIIMBOGNXHQVGW-UHFFFAOYSA-M

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Descrição geral

Sodium bicarbonate (NaHCo3) is a commonly used alkaline buffering agent(5) in laboratories. The addition of this buffer to the cell culture medium aids in maintaining the appropriate pH by using the carbon dioxide present in the incubator. In addition to its buffering properties, Sodium bicarbonate also acts as a source of sodium and aids in sodium gluconate synthesis during the fermentation process.

Aplicação

Sodium bicarbonate solution has been used:
  • as a component of Hanks′ buffered salt solution (HBSS) and recording medium for culturing of tissue explants from rats or mice[1]
  • as a component of Dulbecco′s modified Eagle′s medium (DMEM) to culture mice tissue explants[2]
  • as a component of Minimum Essential Media (MEM) to study the cytotoxicity against human colorectal adenocarcinoma cells and mammary gland adenocarcinoma[3]
  • to prepare collagen mix droplets for 3D collagen sprouting assay[4]
  • to prepare collagen matrix for rat epidermal keratinocyte (REK) organotypic culture[5]

Nota de preparo

Prepared in cell culture grade water.

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

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Certificados de análise (COA)

Lot/Batch Number
RNBM0107
RNBL7713
RNBL6582
RNBL5644
RNBL5422

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Rat epidermal keratinocyte (REK) Organotypic culture (ROC) is an epidermis model that is robust and inexpensive to develop and maintain, and it has in previous studies been shown to have permeability characteristics close to those of human skin. Here, we
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Cell cycle (Georgetown, Tex.), 17(9), 1056-1067 (2018-06-14)
Precise acid-base homeostasis is essential for maintaining normal cell proliferation and growth. Conversely, dysregulated acid-base homeostasis, with increased acid extrusion and marked extracellular acidification, is an enabling feature of solid tumors, yet the mechanisms through which intra- and extracellular pH
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