I1284
Isopropyl β-D-thiogalactopyranoside solution
ReadyMade IPTG solution for Blue-white screening
Sinônimo(s):
IPTG solution
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About This Item
Fórmula empírica (Notação de Hill):
C9H18O5S
Número CAS:
Peso molecular:
238.30
Beilstein:
4631
Número MDL:
Código UNSPSC:
12352200
ID de substância PubChem:
NACRES:
NA.52
Produtos recomendados
grau
for molecular biology
Nível de qualidade
descrição
non-ionic
forma
liquid
peso molecular
238.3
Condições de expedição
dry ice
temperatura de armazenamento
−20°C
cadeia de caracteres SMILES
CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O
InChI
1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
chave InChI
BPHPUYQFMNQIOC-NXRLNHOXSA-N
Descrição geral
Isopropyl thiogalactose (IPTG) is a sugar derivative widely used for the induction of the expression of recombinant proteins in E. coli. This expression system requires that the gene of interest is cloned in plasmids containing the Lac promoter. IPTG Ready Made is a 0.2 μm filter sterilized solution of 200 mM IPTG. This reagent remains liquid at its storage temperature (-20 °C), thus avoiding freeze-thaw cycles and providing a user-friendly solution.
Aplicação
Isopropyl β-D-thiogalactopyranoside solution is:
- used as an inducer for the expression of single domain antibodies(sdAbs) in E coli and bioactive peptides
- used in its half-life and decay studies
- suitable for induction of recombinant protein expression in systems controlled by the lac repressor
Ações bioquímicas/fisiológicas
Isopropyl β-D-thiogalactopyranoside binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner. This binding allows the transcription of genes in the lac operon, such as the gene coding for β--galactosidase. The β-galactosidase gene is transcribed and monosaccharides are the product. Since Isopropyl β-D-thiogalactopyranoside cannot be metabolized by E. coli its concentration remains constant. Therefore, it is not a variable in the experiment.
Princípio
Isopropyl β-D-thiogalactopyranoside binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner. This binding allows the transcription of genes in the lac operon, such as the gene coding for β-galactosidase. The β-galactosidase gene is transcribed and monosaccharides are the product.
produto relacionado
Nº do produto
Descrição
Preços
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Certificados de análise (COA)
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