B2904

Bluo-Gal

reagent for selection of recombinant bacterial clones

• Sinônimo(s): 5-Bromo-3-indolyl β-D-galactopyranoside, substrate for β-galactosidase, Bluo-Gal
• Fórmula empírica (Notação de Hill): C₁₄H₁₆BrNO₆
• Número CAS: 97753-82-7
• Peso molecular: 374.18
• Beilstein: 1550140
• Número MDL: MFCD00063691
• Código UNSPSC: 12352200
• ID de substância PubChem: 24891417
• NACRES: NA.85

Propriedades

• grau: for molecular biology
• Nível de qualidade: 200
• esterilidade: non-sterile
• Ensaio: ≥98% (HPLC)
• Formulário: powder
• técnica(s): nucleic acid detection: suitable
• solubilidade: DMF: soluble
• adequação: suitable for substrate for β-galactosidase
• temperatura de armazenamento: −20°C
• cadeia de caracteres SMILES: OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O
• InChI: 1S/C14H16BrNO6/c15-6-1-2-8-7(3-6)9(4-16-8)21-14-13(20)12(19)11(18)10(5-17)22-14/h1-4,10-14,16-20H,5H2/t10-,11+,12+,13-,14-/m1/s1
• chave InChI: LINMATFDVHBYOS-MBJXGIAVSA-N

Descrição

Descrição geral

Bluo-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Bluo-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Aplicação

Bluo-Gal is suitable for use in selection of recombinant bacterial colonies with the lac⁺ phenotype. Bluo-Gal is suitable for use as β-galactosidase substrate in the staining of skin tissues for electron microscopy analysis,[1] Dictyostelium samples[2] and in the staining of vena cava of adult mice.[3]

Chromogenic substrate suitable for identification of lac⁺ bacterial colonies. An alternative to 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal), producing a darker blue color.

Princípio

When Bluo-Gal is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of Bluo-Gal turn a deeper blue color more intense than X-Gal, allowing for easy differentiation between lac+ and lac- colonies.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)