16669

Magenta-Gal

reagent for selection of recombinant bacterial clones

• Sinônimo(s): 5-Bromo-6-chloro-3-indolyl-β-D-galactopyranoside, Mag-Gal
• Fórmula empírica (Notação de Hill): C₁₄H₁₅BrClNO₆
• Número CAS: 93863-88-8
• Peso molecular: 408.63
• Beilstein: 1552602
• Número MDL: MFCD00210022
• Código UNSPSC: 12352200
• ID de substância PubChem: 329751317

Propriedades

• grau: for molecular biology
• Nível de qualidade: 100
• esterilidade: non-sterile
• Ensaio: ≥98.0% (HPLC)
• Formulário: powder
• solubilidade: DMF: soluble; DMSO: soluble
• adequação: suitable for β-galactosidase test
• temperatura de armazenamento: −20°C
• cadeia de caracteres SMILES: OC[C@H]1O[C@@H](Oc2c[nH]c3cc(Cl)c(Br)cc23)[C@H](O)[C@@H](O)[C@@H]1O
• InChI: 1S/C14H15BrClNO6/c15-6-1-5-8(2-7(6)16)17-3-9(5)22-14-13(21)12(20)11(19)10(4-18)23-14/h1-3,10-14,17-21H,4H2/t10-,11-,12+,13-,14-/m1/s1
• chave InChI: CHRVKCMQIZYLNM-RKQHYHRCSA-N

Descrição

Descrição geral

Magenta-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Magenta-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Aplicação

Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. Also suitable for use immunoblotting and immunocytochemical assays.

Princípio

When Magenta-Gal is hydrolyzed by β-galactosidase, the resulting product will form a red precipitate. Lac+ colonies grown in the presence of Magenta-Gal turn an intense red color, allowing for easy differentiation between lac+ and lac- colonies.

Informações de segurança

• Código de classe de armazenamento: 13 - Non Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)