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GE17-0969-01

IgG Sepharose™ 6 Fast Flow

Cytiva 17-0969-01, pack of 10 mL

Sinônimo(s):

IgG Column

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About This Item

Código UNSPSC:
41106500
NACRES:
NA.56
Preço e disponibilidade não estão disponíveis no momento.

ligand

human IgG

embalagem

pack of 10 mL

fabricante/nome comercial

Cytiva 17-0969-01

condição de armazenamento

(20% Ethanol, 50 mM K2PO4, 1mM MgCl2 pH 7.2 in 20% Ethanol)

Matriz

highly cross-linked 6% agarose

diâmetro médio

90 μm

cleaning in place

3-12

Faixa de trabalho

3-10

capacidade

~3 mg binding capacity(Protein A)

temperatura de armazenamento

2-8°C

Categorias relacionadas

Descrição geral

lgG Sepharose™ 6 Fast Flow, from Cytiva, is based on the rigid Sepharose™ 6 Fast Flow matrix, with human IgG covalently coupled to it. Medium allows high flow rates to be used for rapid and convenient single step purification of protein.

lgG Sepharose™ 6 Fast Flow, from Cytiva, is based on the rigid Sepharose™ 6 Fast Flow matrix, with human IgG covalently coupled to it. The improved mechanical characteristics of this Fast Flow medium allows high flow rates to be used for rapid and convenient single step purification of protein A fusion protein conjungates produced in prokaryotic expression systems.

The mechanical characteristics of this fast flow medium allows high flow rates to be used for rapid and convenient single step purification of protein A fusion protein conjungates produced in prokaryotic expression systems.

Características e benefícios

  • IgG coupled to the highly cross-linked 6% agarose matrix Sepharose™ 6 Fast Flow, designed for the rapid purification of protein A and protein A fusion conjugates
  • Binds at least 2 mg protein A/mL medium
  • Suitable for tandem affinity purification (TAP) of protein complexes

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informações legais

Sepharose is a trademark of Cytiva

Pictogramas

Flame
GHS02

Palavra indicadora

Warning

Frases de perigo

H226

Código de classe de armazenamento

3 - Flammable liquids

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Certificados de análise (COA)

Lot/Batch Number

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Artigos

Performing a Separation with IgG Sepharose 6 Fast Flow

Perform a separation with IgG Sepharose 6 Fast Flow from Cytiva, an Affinity Chromatography product for purification of recombinant fusion proteins containing a protein A tail.

Characteristics of Dextrin Sepharose® High Performance Products

This page shows the characteristics of Dextrin Sepharose High Performance products from Cytiva.

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocolos

Purification of Protein A-Tagged Proteins

This page shows how to purify protein A-tagged proteins using Cytiva products.

Pull-down assays

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.

Column Packing and Preparation Protocol for Affinity Chromatography

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

Conteúdo relacionado

Técnicas de “pull-down” de proteínas

Investigue técnicas de interação entre proteínas com ensaios de pull-down, utilizando métodos de afinidade, pull-down de GST e coimunoprecipitação.

Protein Pull-Down Techniques

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

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