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GE17-0618-01

Protein G Sepharose 4 Fast Flow

Cytiva 17-0618-01, pack of 5 mL

Sinônimo(s):

Fast Flow resin, Antibody purification resin, IgG purification resin

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About This Item

Código UNSPSC:
41106500
NACRES:
NA.56

ligand

recombinant protein G lacking albumin-binding region

embalagem

pack of 5 mL

fabricante/nome comercial

Cytiva 17-0618-01

condição de armazenamento

(20% Ehtanol)

matriz

4% cross-linked agarose

diâmetro médio

90 μm (d50v)

cleaning in place

2-10

working range

3-9

adequação

suitable for bioprocess medium

temperatura de armazenamento

2-8°C

Descrição geral

Protein G Sepharose 4 Fast Flow is recombinant protein G coupled to Sepharose 4 Fast Flow.

Protein G Sepharose 4 Fast Flow has recombinant protein G immobilized by the cyanogen bromide (CNBr) method to Sepharose 4 Fast Flow. Protein G exhibit binding specificities that complement Protein A media and binds to the Fc region of IgG from a variety of mammalian species. Protein G Sepharose 4 Fast Flow may be used to isolate and purify classes, subclasses and fragments of immunoglobulins from any biological fluid or cell culture medium.

As member of the BioProcess media range, Protein G Sepharose 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Características e benefícios

  • Binding specificities that complement Protein A media.
  • Binds a broad range of IgG species and subclasses.
  • Multi-point attachment minimizes ligand leakage.
  • Used in a range of research applications.

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informações legais

Sepharose is a trademark of Cytiva

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Palavra indicadora

Warning

Frases de perigo

Código de classe de armazenamento

3 - Flammable liquids


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Artigos

This page shows a comparison of the relative binding strengths of protein G and protein A to different immunoglobulins.

Purify monoclonal or polyclonal IgG from serum, cell culture supernatant or ascitic fluid using the HiTrap Protein G HP from Cytiva, an affinity-exclusion chromatography product containing Sepharose-immobilized Protein G.

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

This page describes efficient column packing and preparation for affinity chromatography of antibodies.

Ver tudo

Protocolos

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.

This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from Cytiva.

This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from Cytiva.

Conteúdo relacionado

Ensaios, reagentes e protocolos de “pull-down” para investigar interações proteicas in vitro usando métodos de afinidade ou de pull-down com GST, purificação por afinidade em tandem (TAP) e de coimunoprecipitação.

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

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