P3296
Protein G Sepharose™, Fast Flow
recombinant, expressed in E. coli, aqueous ethanol suspension
Sinônimo(s):
Protein G-Agarose, Fast Flow from Streptococcus sp.
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About This Item
Produtos recomendados
recombinante
expressed in E. coli
Nível de qualidade
forma
aqueous ethanol suspension
classe(s) química(is) do analito
proteins (Immunoglobulins of various mammalian species)
Extensão da rotulagem
~2 mg per mL
técnica(s)
affinity chromatography: suitable
matriz
Sepharose 4B Fast Flow
ativação da matriz
cyanogen bromide
ligação da matriz
amino
espaçador de matriz
1 atom
temperatura de armazenamento
2-8°C
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Descrição geral
Protein G is an immunoglobulin (IgG)-binding bacterial cell wall protein isolated from group G streptococcal strain, G-148. This protein can be extracted from the cells by papain digestion and purified by the sequential use of ion-exchange chromatography on DEAE-Sephadex, affinity chromatography on Sepharose-coupled human IgG, and gel chromatography on Sephadex G-200. The binding between protein G and various polyclonal and monoclonal IgG is basically pH dependent between 2.8 and 10, with the strongest binding at pH 4 and 5, and weakest at pH 10. It acts as a powerful reagent for the detection of IgG.
Protein G is an immunoglobulin (IgG)-binding bacterial cell wall protein isolated from group G streptococcal strain, G-148. This protein can be extracted from the cells by papain digestion and purified by the sequential use of ion-exchange chromatography on DEAE-Sephadex, affinity chromatography on Sepharose-coupled human IgG, and gel chromatography on Sephadex G-200. The binding between protein G and various polyclonal and monoclonal IgG is basically pH dependent between 2.8 and 10, with the strongest binding at pH 4 and 5, and weakest at pH 10. It acts as a powerful reagent for the detection of IgG.
P3296-5Ml′s updated product number is GE17-0618-01
P3296-5Ml′s updated product number is GE17-0618-01
Aplicação
Protein G-Sepharose™ is used in affinity chromatography, protein chromatography, antibody purification and characterization, immunoaffinity matrices, protein A, G and L resins, protein interaction, and purification and detection. Protein G-Sepharose™ has been used to develop a strategy to confirm the presence of anti-erythropoietin neutralizing antibodies in human serum as well as to compare methods for depletion of albumin and IgG from equine serum.
forma física
Suspension in 20% ethanol
Nota de preparo
Prepared with recombinant streptococcal Protein G from which the albumin-binding region has been genetically deleted
Informações legais
Sepharose is a trademark of Cytiva
produto relacionado
Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Classificações de perigo
Flam. Liq. 3
Código de classe de armazenamento
3 - Flammable liquids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
115.0 °F - closed cup
Ponto de fulgor (°C)
46.1 °C - closed cup
Certificados de análise (COA)
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Protocolos
Techniques for protein antigen molecular weight determination, protein interactions, enzymatic activity, and post-translational modifications.
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