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G9768

Sigma-Aldrich

Guanosine 5′-triphosphate–Agarose

aqueous glycerol suspension

Sinônimo(s):

5′-GTP agarose

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About This Item

Número MDL:
MFCD00171655
Código UNSPSC:
41106500
NACRES:
NA.56

fonte biológica

bacterial (CORYNEBACTERIUM SP)
enzyme from Porcine brain
plant (Sea weed)
yeast

forma

aqueous glycerol suspension

Extensão da rotulagem

1-5 μmol per mL

matriz

cross-linked 4% beaded agarose

ativação da matriz

cyanogen bromide

ligação da matriz

ribose hydroxyls

espaçador de matriz

11 atoms (adipic acid dihydrazide)

temperatura de armazenamento

−20°C

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Aplicação

Guanosine 5′-triphosphate-agarose (5′-GTP agarose) is an agarose conjugate in aqueous glycerol suspension used in affinity chromatography, for purification of guanosine-binding proteis

forma física

Guanosine 5′-triphosphate–Agarose has been used:
  • in chromatographic column for the purification of a translocation stimulating factor
  • in GTP-Agarose pull down assays to measure the levels of GTP-bound RHEB (Ras homolog enriched in brain)
  • in GTP-Binding assay
  • for the depletion of GTP binding proteins in cytosol of rat liver
Suspension in 50% glycerol containing 0.25 M NaCl

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

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T E Allen et al.
Molecular and biochemical parasitology, 65(2), 233-245 (1994-06-01)
The hypoxanthine-guanine phosphoribosyltransferase (HGPRT) enzyme in Trypanosoma cruzi is a rational target for the treatment of Chagas disease. To evaluate the T. cruzi HGPRT in detail, the HGPRT gene (hgprt) was cloned from a genomic library of T. cruzi DNA
Possible role of both the alpha and beta gamma subunits of the heterotrimeric G protein, Gs, in transcytosis of the polymeric immunoglobulin receptor
Bomsel M and Mostov KE
The Journal of Biological Chemistry, 268, 25824-25835 (1993)
Reiaz Ul Rehman et al.
Plant & cell physiology, 49(5), 751-766 (2008-04-04)
The regulatory functions of Rab proteins in membrane trafficking lie in their ability to perform as molecular switches that oscillate between a GTP- and a GDP-bound conformation. The role of tomato LeRab11a in secretion was analyzed in tobacco protoplasts. Green
Arsenite stress down-regulates phosphorylation and 14-3-3 binding of leucine-rich repeat kinase 2 (LRRK2), promoting self-association and cellular redistribution
Mamais A, et al.
The Journal of Biological Chemistry, 289, 21386-21400 (2014)
P E Glaser et al.
Biochemistry, 34(38), 12193-12203 (1995-09-26)
Recently we demonstrated that the unique stereoelectronic relationships inherent in the structure of plasmenylethanolamine facilitate membrane fusion, and we postulated the existence of a membrane fusion protein which could exploit the propensity of plasmenylethanolamine molecular species to adapt an inverted
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