10106399001

GTP

>90% (HPLC), crystals, pkg of 250 mg

• Sinônimo(s): Guanosine 5′-triphosphate sodium salt hydrate, 5′-GTP-Na₂
• Fórmula empírica (Notação de Hill): C₁₀H₁₆N₅O₁₄P₃ · xNa⁺ · yH₂O
• Número CAS: 56001-37-7
• Peso molecular: 523.18 (anhydrous free acid basis)
• Beilstein: 4113439
• Número MDL: MFCD00149227
• Código UNSPSC: 41106305
• ID de substância PubChem: 329748894

Propriedades

• descrição: Disodium salt
• Nível de qualidade: 100
• Ensaio: >90% (HPLC)
• Formulário: crystals
• peso molecular: M_r 523.2 (GTP); M_r 567.1 (GTP-Na₂)
• embalagem: pkg of 250 mg
• fabricante/nome comercial: Roche
• condição de armazenamento: avoid repeated freeze/thaw cycles
• cadeia de caracteres SMILES: O.O.[Na+].[Na+].NC1=Nc2c(ncn2[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)[C@@H](O)[C@H]3O)C(=O)N1
• InChI: 1S/C10H16N5O14P3.2Na.2H2O/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21;;;;/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18);;;2*1H2/q;2*+1;;/p-2/t3-,5-,6-,9-;;;;/m1..../s1
• chave InChI: HPLNHYUZFVUXFJ-ZVQJTLEUSA-L

Descrição

Descrição geral

GTP functions as a carrier of phosphates and pyrophosphates involved in channeling chemical energy into specific biosynthetic pathways. It also serves as an energy-rich precursor of mononucleotide units in the enzymatic biosynthesis of DNA and RNA.[1] GTP is involved in the initiation of peptide synthesis during the binding of formylmethionyl-tRNA (F-met-tRNA) to the ribosome.[2][3] It is also involved in polypeptide chain elongation.[4] GTP is hydrolyzed by tubulin and its hydrolysis is accompanied by microtubule assembly.[5]

GTP is supplied as the disodium salt in crystal form.

Aplicação

GTP, disodium salt, is required as a coenzyme for protein biosynthesis in a cell-free system. It has been used in FtsZ polymerization assay[6] and in the preparation of pipette solution for the electrophysiological recordings of the whole-cell membrane currents.[7]

Nota de preparo

Activator: EDTA prevents the formation of enzyme aggregates. Phosphate stimulates the NADH-dependent reaction.
Storage conditions (working solution): Aqueous solutions (pH 7.0) are stable at 2 to 8 °C or frozen for approx. one week, for reasons of hydrolysis pH should not be < 3.

If neutralized with water preparation can be stored frozen at -15 to -25 °C in small portions, one sample should thawed only once and should be used within the next one or two days (stored at 2 to 8 °C). Preparation can also be dissolved in a buffer pH 7-8 without neutralization (buffer concentration should be high enough that pH is not changed by addition of those substances); solution can be stored frozen.

Investigations on the storage temperature 2 to 8 °C compared to -15 to -25 °C showed no difference.

Armazenamento e estabilidade

Store at 2 to 8 °C. (Store dry!)

Nota de análise

Purity: 76% GTP (enzymatic), >90 area% (HPLC), 9% Na⁺, 8% H2O

Outras notas

For life science research only. Not for use in diagnostic procedures.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable