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G2751

Sigma-Aldrich

Glutamic-Oxalacetic Transaminase from porcine heart

Type I, ammonium sulfate suspension, 200-500 units/mg protein

Sinônimo(s):

L-Aspartate:2-oxoglutarate aminotransferase, Aspartate Aminotransferase, GOT

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1000 UNITS
R$ 1.404,00
2000 UNITS
R$ 2.626,00
10000 UNITS
R$ 10.314,00

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1000 UNITS
R$ 1.404,00
2000 UNITS
R$ 2.626,00
10000 UNITS
R$ 10.314,00

About This Item

Número CAS:
Número da licença da enzima:
Número CE:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54

R$ 1.404,00


Previsão de entrega em01 de abril de 2025


Solicite uma grande encomenda

tipo

Type I

Nível de qualidade

Formulário

ammonium sulfate suspension

atividade específica

200-500 units/mg protein

atividade externa

glutamic-pyruvic transaminase ≤0.03%
lactic dehydrogenase ≤0.01%
malic dehydrogenase ≤0.01%

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

Descrição geral

Research area: Cellsignaling. Glutamic-Oxalacetic Transaminase is found in all tissues and is predominant in the liver and skeletal muscle.[1] It is a prototype of fold-type I pyridoxal 5′-phosphate (PLP)-enzymes. Glutamic-oxalacetic transaminase is a homodimer containing large and small domains in each subunit.[2]

Aplicação

Glutamic-OxalaceticTransaminase from porcine heart is suitable for the preparation ofphenylalanine amino acid. The immobilized form of this enzyme maybe used incomparison studies between soluble and immobilized forms in terms of reactionefficiency and stability.
Glutamic-Oxalacetic Transaminase from porcine heart has been used:
  • in an enzyme-coupled assay for evaluating L-asparaginase enzyme activity[3]
  • in microtiter plate format enzyme-based assay to estimate malic acid content in berry samples[4]
  • as a supplement in homogenization buffers for the isolation of heart mitochondrial membranes in the presence of oxaloacetate (OAA)-depleting system[5]

Ações bioquímicas/fisiológicas

Glutamic-oxalacetic transaminase or aspartate aminotransferase (AAT) catalyzes the interconversion of L-aspartate and α-ketoglutarate with oxalacetate and L-glutamate. This reversible transaminase reaction is dependent on pyridoxal 5′-phosphate (PLP). Glutamic-oxalacetic transaminase maintains the nitrogen currency for metabolism by generating L-glutamate.[6] Elevated serum levels of glutamic-oxalacetic transaminase are indicated in myocardial infarction, liver diseases, and some renal diseases.[7]

Definição da unidade

One unit will convert 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.5 at 37 °C, in the presence of L-aspartic acid. One unit is equivalent to ~2,000O.D. (Karmen) units at 25 °C.

forma física

Suspension in 3.0 M (NH4)2SO4 containing 0.05 M maleate and 2.5 mM α-ketoglutarate, pH 6.0

Nota de análise

Protein determined by biuret.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


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Artigos

Instructions for working with enzymes supplied as ammonium sulfate suspensions

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