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F7425

Millipore

ANTI-FLAG®

ANTI-FLAG® antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Sinônimo(s):

Anti-ddddk, Anti-dykddddk

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0.2 MG
R$ 2.646,00

R$ 2.646,00

Previsão de entrega em27 de março de 2025

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0.2 MG
R$ 2.646,00

About This Item

Número MDL:
MFCD02262912
Código UNSPSC:
12352203
NACRES:
NA.32
clone:
polyclonal
application:
DB
IF
IP
WB CL
reatividade de espécies:
all
técnica(s):
dot blot: 1-2.5 μg/mL
immunoprecipitation (IP): 4-8 μg using amino terminal FLAG-BAP fusion protein from E. coli crude lysate
indirect immunofluorescence: 5-10 μg/mL using 293T cells transfected with a plasmid encoding FLAG-JNK
western blot (chemiluminescent): 1-2.5 μg/mL using an E. coli periplasmic extract expressing an N-terminal FLAG fusion protein
citations:
3023

R$ 2.646,00

Previsão de entrega em27 de março de 2025

Solicite uma grande encomenda

fonte biológica

rabbit

Nível de qualidade

200

conjugado

unconjugated

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

Formulário

buffered aqueous solution

reatividade de espécies

all

concentração

~0.8 mg/mL

técnica(s)

dot blot: 1-2.5 μg/mL
immunoprecipitation (IP): 4-8 μg using amino terminal FLAG-BAP fusion protein from E. coli crude lysate
indirect immunofluorescence: 5-10 μg/mL using 293T cells transfected with a plasmid encoding FLAG-JNK
western blot (chemiluminescent): 1-2.5 μg/mL using an E. coli periplasmic extract expressing an N-terminal FLAG fusion protein

Isotipo

IgG

sequência de imunogênio

DYKDDDDK

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

Categorias relacionadas

Descrição geral

O anticorpo policlonal de coelho com afinidade ANTI-FLAG reconhece o epítopo FLAG localizado nas proteínas de fusão FLAG. Este anticorpo reage com proteínas de fusão FLAG N-terminais, com Met N-terminal e C-terminais.
O epítopo FLAG é uma proteína de oito aminoácidos com um sítio de clivagem para enteroquinase.[1][2]

Imunogênio

FLAG; sequência de peptídeo DYKDDDDK

Aplicação

O anticorpo reconhece o epítopo FLAG localizado nas proteínas de fusão marcadas com FLAG na extremidade N-terminal ou C-terminal, com aplicação de ensaios dot blot e de imunotransferência[3][4], imunoprecipitação[3] e imunocitoquímica.
Saiba mais detalhes sobre o produto em nosso portal de aplicações FLAG®”.

Ações bioquímicas/fisiológicas

O epítopo FLAG tem um papel essencial na purificação de proteínas de fusão por imunoafinidade.[1]

forma física

Solução em solução salina tamponada com fosfato 0,01 M, pH 7,4, contendo albumina sérica bovina 1% e azida sódica 15 mM

Nota de preparo

Purificado por cromatografia de afinidade em uma coluna com o peptídeo imunizante.

Outras notas

Vencedor do Prêmio CiteAb 2024 para Fornecedor de Sucesso em Pesquisa sobre Parkinson′

Informações legais

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

nwg

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Escolha uma das versões mais recentes:

Certificados de análise (COA)

Lot/Batch Number
0000395474
0000395474
0000252651
0000236831
0000160280

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Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Ryota Sato et al.
Frontiers in microbiology, 10, 1857-1857 (2019-08-29)
The envelope proteins of influenza A virus, hemagglutinin (HA) and neuraminidase (NA), play critical roles in viral entry to host cells and release from the cells, respectively. After protein synthesis, they are transported from the trans-Golgi network (TGN) to the
Martin S Taylor et al.
The Journal of biological chemistry, 288(45), 32211-32228 (2013-09-21)
Ghrelin O-acyltransferase (GOAT) is a polytopic integral membrane protein required for activation of ghrelin, a secreted metabolism-regulating peptide hormone. Although GOAT is a potential therapeutic target for the treatment of obesity and diabetes and plays a key role in other
Makoto Miyazawa et al.
The Journal of biological chemistry, 286(22), 19191-19203 (2011-04-12)
The molecular chaperone prefoldin (PFD) is a complex comprised of six different subunits, PFD1-PFD6, and delivers newly synthesized unfolded proteins to cytosolic chaperonin TRiC/CCT to facilitate the folding of proteins. PFD subunits also have functions different from the function of
Ecco Staller et al.
Journal of virology, 93(17) (2019-06-21)
ANP32 proteins have been implicated in supporting influenza virus replication, but most of the work to date has focused on the ability of avian Anp32 proteins to overcome restriction of avian influenza polymerases in human cells. Using a CRISPR approach
Dorothee A Vogt et al.
PLoS pathogens, 9(4), e1003302-e1003302 (2013-04-18)
The nonstructural protein NS5A has emerged as a new drug target in antiviral therapies for Hepatitis C Virus (HCV) infection. NS5A is critically involved in viral RNA replication that takes place at newly formed membranes within the endoplasmic reticulum (membranous
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1 Review This action will navigate to reviews.

Questions

1–9 of 9 Questions  

  1. Can this AB (F7425) used for mouse 30um thick section. mouse will be perfused with 4% PFA and than cryopreserved. thanks

    1 answer

    1. This product has been recommended for use in immunofluorescence and indirect immunofluorescence at 5-10 μg/mL using 293T cells transfected with a plasmid encoding FLAG-JNK. It has not been tested for use in IHC applications. The end-user will need to optimize the assay. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/207/353/f7425dat-ms.pdf

      In general, antibodies work well on 5-15 uM sections, for 30 uM sections, blocking /permeabilization steps and antibody dilution, the incubation time will need to be optimized by end user. Please see the links below to review an alternative anti-Flag antibody that may be more suitable for this application. Note that this product is a mouse monoclonal antibody:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/175/747/f1804bul-ms.pdf
      https://www.sigmaaldrich.com/product/sigma/f1804

      Helpful?

  2. Is there a recommended isotype control for this antibody?

    1 answer

    1. Any rabbit IgG such as product I5006 would be an appropriate isotype control for this antibody.

      Helpful?

  3. What are the positive controls that can be used with Product F7425, ANTI-FLAG® from rabbit?

    1 answer

    1. The positive controls that can be used are as follows:Amino-terminal FLAG-BAP fusion protein, Product No. P7582Carboxy-terminal FLAG-BAP fusion protein, Product No. P7457Amino-terminal Met-FLAG-BAP fusion protein, Product No. P5975.

      Helpful?

  4. How to get rid of background when using Product F7465, ANTI-FLAG® from rabbit,?

    1 answer

    1. To reduce non-specific background on tissue staining, a small amount of Triton X-100 (Product No. X100 or T8787), can be added to the wash buffers and antibody incubation buffers. Try a Triton X-100 concentration of 0.1% to start. Tween 20 (Product No. P5927) can also be used.

      Helpful?

  5. What is the concentration of Product F7425, ANTI-FLAG® from rabbit?

    1 answer

    1. The concentration is 0.9 mg protein per ml.

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  6. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  7. Is Product F7425, ANTI-FLAG® from rabbit, a monoclonal or polyclonal?

    1 answer

    1. F7425 is a polyclonal antibody from rabbit.

      Helpful?

  8. Is there any references for the use of Product F7465, ANTI-FLAG® from rabbit, in immunohistochemistry or western blotting?

    1 answer

    1. K. Schafer and T. Braun, Monoclonal Anti-FLAG Antibodies React with a New Isoform of Rat Mg++ Dependent Protein Phosphatase. Biochemical and Biophysical Research Communications, 207, 708.

      Helpful?

  9. How much Product F7425, ANTI-FLAG® from rabbit, should be used in western blotting and ELISA applications?

    1 answer

    1. For a Western blot from a PAGE run (preferably) under denaturing conditions, a 1:400 dilution of this antibody should be used.  A 1-10 μg/ml concentration should be used when using thie antibody in an ELISA application.

      Helpful?

Reviews

Active Filters

  1. Nottingham
    • Review 1
    • Votes 0
    5 out of 5 stars.

    Western blotting

    I HAVE USED THIS ANTIBODY ALOT DURING MY RESEARCH. AND IT HAS WORKED PERFECTLY.

    Helpful?

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