FLAGIPT1
FLAG® Immunoprecipitation Kit
Sinônimo(s):
Anti-ddddk, Anti-dykddddk
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About This Item
Código UNSPSC:
12352200
NACRES:
NA.32
Produtos recomendados
uso
kit sufficient for 50 reactions
Nível de qualidade
classe(s) química(is) do analito
proteins (FLAG® Affinity Gels)
técnica(s)
immunoprecipitation (IP): suitable
Condições de expedição
wet ice
temperatura de armazenamento
−20°C
Descrição geral
Sigma′s FLAG Immunoprecipitation kit allows a rapid and efficient immunoprecipitation and elution of an active FLAG-tagged protein. The immunoprecipitation is performed with the ANTI-FLAG M2 affinity gel, which is a highly specific monoclonal antibody covalently attached to agarose resin. The use of affinity resin allows an efficient binding of FLAG-tagged proteins without the need for preliminary steps and calibrations. The immunoprecipitated FLAG-tagged proteins can be efficiently eluted from the resin with acidic conditions or by competition with FLAG peptide. The immunoprecipitated proteins can be detected for their size, post-translational modifications, and interactions on gel electrophoresis, and by activity assays.
Aplicação
Learn more product details in our FLAG® application portal.
Características e benefícios
- Utilizes highly specific ANTI-FLAG M2 affinity gel
- No preliminary steps or calibrations
- 3X FLAG™ peptide provides efficient and gentle elution through direct competition
Outras notas
All prodedures to be performed at 2-8°C.
Informações legais
3xFLAG is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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Frases de perigo
Declarações de precaução
Classificações de perigo
Aquatic Chronic 3 - Eye Irrit. 2
Código de classe de armazenamento
10 - Combustible liquids
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