F4042
Monoclonal ANTI-FLAG® M5 antibody produced in mouse
clone M5, purified immunoglobulin, buffered aqueous solution
Sinônimo(s):
Anti-ddddk, Anti-dykddddk
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About This Item
Produtos recomendados
fonte biológica
mouse
conjugado
unconjugated
forma do anticorpo
purified immunoglobulin
tipo de produto de anticorpo
primary antibodies
clone
M5, monoclonal
forma
buffered aqueous solution
purificado por
using Protein A
reatividade de espécies
all
concentração
2-5 mg/mL
técnica(s)
western blot (chemiluminescent): 10 μg/mL
Isotipo
IgG1
sequência de imunogênio
DYKDDDDK
Condições de expedição
dry ice
temperatura de armazenamento
−20°C
Descrição geral
Monoclonal ANTI-FLAG® M5 is a mouse antibody that binds to N-terminal Met-FLAG fusion proteins. It is useful for detection of N-terminal Met-FLAG fusion proteins expressed in mammalian and Drosophilae cells.
Method of purification - Protein A
Method of purification - Protein A
Imunogênio
FLAG; peptide sequence DYKDDDDK
Aplicação
Monoclonal ANTI-FLAG® M5 antibody produced in mouse has been used in western blotting and immunofluorescence.
Binds the FLAG peptide only when it is located at the amino terminus preceded by a methionine. Binding is not Ca2+-dependent. Useful for detecting cytoplasmically expressed Met-FLAG fusion proteins in mammalian crude cell extracts, but not recommended for fusion proteins expressed in E. coli.
forma física
Solution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v)
Nota de preparo
Dilute the antibody to 10 mg/mL in Tris buffered saline (TBS): 0.05 M Tris, 0.15 M NaCl, pH 7.4.
Outras notas
Antibody is not calcium dependent.
Informações legais
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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produto relacionado
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
nwg
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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