E8145

Ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid tetrasodium salt

≥97%

• Sinônimo(s): EGTA tetrasodium, Egtazic acid tetrasodium, Ethylene-bis(oxyethylenenitrilo)tetraacetic acid tetrasodium
• Fórmula empírica (Notação de Hill): C₁₄H₂₀N₂O₁₀Na₄
• Número CAS: 13368-13-3
• Peso molecular: 468.28
• Número MDL: MFCD00274420
• Código UNSPSC: 12352202
• ID de substância PubChem: 24894667
• NACRES: NA.77

Propriedades

• Ensaio: ≥97%
• técnica(s): RNA purification: suitable
• solubilidade: H₂O: soluble 10%, clear, colorless
• aplicação(ões): life science and biopharma
• cadeia de caracteres SMILES: [Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CCOCCOCCN(CC([O-])=O)CC([O-])=O)CC([O-])=O
• InChI: 1S/C14H24N2O10.4Na/c17-11(18)7-15(8-12(19)20)1-3-25-5-6-26-4-2-16(9-13(21)22)10-14(23)24;;;;/h1-10H2,(H,17,18)(H,19,20)(H,21,22)(H,23,24);;;;/q;4*+1/p-4
• chave InChI: BAOGCDPNNOBWQZ-UHFFFAOYSA-J

Descrição

Aplicação

A chelating agent useful for the determination of calcium in the presence of magnesium.

A chelating agent useful for the determination of calcium in the presence of magnesium. Repeated washing with EGTA-buffer can experimentally deplete cultured cells of Ca²⁺.

EGTA is suitable for use:

• in media for chelating Ca²⁺ to facilitate efficient incorporation of Sr²⁺ into the oocytes[1]
• as a component of phosphatase wash buffer used to wash magnetic beads during dephosphorylation and radiolabeling of RNA segments cross-linked to immunoprecipitated proteins[2]
• as a component of oocyte activation media[3]

Ações bioquímicas/fisiológicas

Ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetra acetic acid tetrasodium salt (EGTA) dissociates mucin complex and is useful in immunohistochemical studies.[4] It suppresses root growth and mitotic index in germinating wheat seeds.[5] It is used as a decalifying agent in root canal biomechanical preparation and is effective compared to EDTA and cyclohexanediaminetetraacetic acid (CDTA).[6]

Ethylene glycol-bis(ß-aminoethyl ether)-N,N,N,N-tetra acetic acid tetrasodium salt (EGTA) is a calcium chelator that binds to Ca²⁺ in the presence of Mg²⁺ at a ratio of 1:1.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)