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E3762

Sigma-Aldrich

pFLAG-CMV™−5a,b,c Expression Vectors

0.5 mg/mL

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About This Item

Número MDL:
MFCD03100886
Código UNSPSC:
12352200

etiqueta

FLAG® tagged

grau

for molecular biology

forma

buffered aqueous solution

concentração

0.5 mg/mL

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

Descrição geral

p-FLAG-CMV-5a, p-FLAG-CMV-5b, and p-FLAG-CMV-5c Expression Vectors used for the expression of C-terminal FLAG® tagged proteins in mammalian cells. pFLAG-CMV-5 a,b,c Expression Vectors are used as shuttle vectors for E. coli and mammalian cells. These vectors facilitate the intracellular expression of C-terminal FLAG-tagged fusion proteins. Transient expression in mammalian cells is driven by the CMV promoter-regulatory region. The SV40 origin of replication results in most efficient replication in mammalian cells expressing the SV40 large T antigen. The a, b, and c vector series makes all three reading frames available for every restriction site in the MCS.

Vector Maps and Sequences

Componentes

  • p-FLAG-CMV-5a Expression Vector (20μg)
  • p-FLAG-CMV-5b Expression Vector (20 μg)
  • p-FLAG-CMV-5c Expression Vector (20 μg)
  • pFLAG-CMV-5a-BAP control plasmid (20 μg)

Informações legais

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
pFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC

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NAD kinases (NADKs) are vital, as they generate the cellular NADP pool. As opposed to three compartment-specific isoforms in plants and yeast, only a single NADK has been identified in mammals whose cytoplasmic localization we established by immunocytochemistry. To understand
Jinlong Wang et al.
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KRAB-containing zinc finger proteins (KZNF) constitute the largest family of transcriptional regulators in humans and play critical roles in normal development and tumorigenesis. However, the function and mechanism of most KZNFs remain unclear. Here, we report that ZNF496, a KZNF

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