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AB0300

Sigma-Aldrich

Alkaline Phosphatase Blue Membrane Substrate Solution

alkaline phosphatase substrate, solution

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About This Item

Código UNSPSC:
12352204
eCl@ss:
42010105
NACRES:
NA.83

Nome do produto

Alkaline Phosphatase Blue Membrane Substrate Solution, sufficient for 400 mL working substrate

Formulário

solution

Nível de qualidade

200

uso

sufficient for 400 mL working substrate

temperatura de armazenamento

2-8°C

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Descrição geral

Alkaline phosphatase, a metalloenzyme is a member of ectonucleotidases family.[1] It is present in bile ducts, bone, intestine, placenta and tumors.[2]

Aplicação

Alkaline Phosphatase Blue Membrane Substrate Solution has been used:
  • to analyse direct alkaline phosphatase (AP) activity[3]
  • to perform alkaline phosphatase assays with colorimetric system[4]
  • to analyse direct alkaline phosphatase (AP) activity of the induced pluripotent stem cells (iPSC) line LND554SV.3[5][6]

Prior to reaction with alkaline phosphatase, the BCIP® reagent is colorless to faint blue and the Nitro BT reagent is yellow. The two-component mixture develops a bluish-purple product with alkaline phosphatase in membrane-type assays. This substrate is not recommended for ELISA assays.

forma física

A 2 component kit sufficient for preparing 400 mL of working substrate.
Supplied as a two-component buffered alkaline phosphatase substrate containing bromochloroindolyl phosphate (BCIP®) and Nitro Blue Tetrazolium (NBT/Thiazolyl Blue/Nitro BT)

Informações legais

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de classe de armazenamento

10 - Combustible liquids

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

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Certificados de análise (COA)

Lot/Batch Number
SLCM0073
SLCL7179
SLCF1959
SLCB1691
SLBZ1825

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The Short Isoform of NF-YA Belongs to the Embryonic Stem Cell Transcription Factor Circuitry
Dolfini D, et al.
Stem Cells, 30(11), 2450-2459 (2012)
Inhibition of alkaline phosphatase: an emerging new drug target
Al-Rashida M and Iqbal J
Mini-Reviews in Medicinal Chemistry, 15(1), 41-51 (2015)
Generation of a human iPSC line from a patient with Leigh syndrome
Galera T, et al.
Stem Cell Research, 16(1), 63-66 (2016)
María Del Carmen Ortuño-Costela et al.
Stem cell research, 23, 188-192 (2017-09-20)
Human iPSC line IISHDOi001-A was generated from fibroblasts of a patient with McArdle disease harbouring the mutation, c.148C>T; p.Arg50Ter, in the PYGM gene. Reprogramming factors Oct3/4, Sox2, Klf4, and c-Myc were delivered using Sendai virus.
Victoria Cerrada et al.
Stem cell research, 40, 101566-101566 (2019-09-12)
Human iPSC line, IISHDOi006-A, was obtained from fibroblasts of a patient with Dominant Optic Atrophy (DOA) carrying a heterozygous mutation in the gene ACO2: c.1999G>A; p.Glu667Lys. Reprogramming factors Oct3/4, Sox2, Klf4, and c-Myc were delivered using a non-integrative methodology that
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Artigos

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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