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A4605

Sigma-Aldrich

Anti-iASPP antibody, Mouse monoclonal

clone LXO49.3, purified from hybridoma cell culture

Sinônimo(s):

Anti-Inhibitory Member of the Apoptosis-stimulating Protein of p53 Family, Anti-RAI, Anti-Rel-associated Inhibitor

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About This Item

Número MDL:
MFCD08705587
Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

200

conjugado

unconjugated

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

LXO49.3, monoclonal

forma

buffered aqueous solution

peso molecular

antigen ~100 kDa

reatividade de espécies

mouse, human

concentração

~2 mg/mL

técnica(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 0.1-0.2 μg/mL using total cell extract of human osteogenic sarcoma (U-2-OS)

Isotipo

IgG1

nº de adesão UniProt

Q8WUF5

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... PPP1R13L(10848)
mouse ... Ppp1r13l(333654)

Descrição geral

Monoclonal Anti-iASPP (mouse IgG1 isotype) is derived from the hybridoma LXO49, produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice immunized with a recombinant protein encoding residues of human iASPP. The ASPP family of proteins contains three members, ASSP1, ASSP2 and iASPP. iASPP is also known as REL associated inhibitor (RAI). iASPP exists as a 100 kDa form and as a 70 kDa form (iASPP/RAI). It is found both in the nucleus and cytoplasm, while iASPP/RAI is mainly cytosolic. PPP1R13L (protein phosphatase 1 regulatory subunit 13 like) codes for iASPP, that is located on human chromosome 19q13.
iASPP is an inhibitory member of the apoptosis-stimulating protein of p53 (ASPP) family. It is an oncoprotein that associates with Ras, E1A and E7 to transform cells.

Aplicação

Monoclonal Anti-iASPP antibody produced in mouse has been used in:
  • immunoblotting
  • immunocytochemistry
  • immunoprecipitation

Mouse monoclonal Anti-iASPP antibody can be used for western blot (0.1-0.2μg/mL using total cell extract of human osteogenic sarcoma), microarray, immunoprecipitation and immunocytochemistry.

Ações bioquímicas/fisiológicas

PPP1R13L (protein phosphatase 1 regulatory subunit 13 like)/iASPP inhibits p53-mediated cell death. It interacts and inhibits NFkB p65 RelA. It may act as a therapeutic target for bladder cancer (BC). It modulates cell proliferation and apoptosis. iASPP plays a key role in breast cancer and acute leukemia.

forma física

Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

nwg

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Ariel M Wilson et al.
PloS one, 9(4), e94175-e94175 (2014-04-10)
The transcription factor p53 mediates the apoptosis of post-mitotic neurons exposed to a wide range of stress stimuli. The apoptotic activity of p53 is tightly regulated by the apoptosis-stimulating proteins of p53 (ASPP) family members: ASPP1, ASPP2 and iASPP. We
The miR-124-p63 feedback loop modulates colorectal cancer growth
Liu K, et al.
Oncotarget, 8(17), 29101-29101 (2017)
Wenjie Ge et al.
Cancer cell, 32(5), 561-573 (2017-10-17)
Reactive oxygen species (ROS) have emerged as important signaling molecules that play crucial roles in carcinogenesis and cytotoxic responses. Nrf2 is the master regulator of ROS balance. Thus, uncovering mechanisms of Nrf2 regulation is important for the development of alternative
A pro-apoptotic function of iASPP by stabilizing p300 and CBP through inhibition of BRMS1 E3 ubiquitin ligase activity
Kramer D, et al.
Cell Death & Disease, 6(2), e1634-e1634 (2015)
Anissa Chikh et al.
The EMBO journal, 30(20), 4261-4273 (2011-09-08)
iASPP, an inhibitory member of the ASPP (apoptosis stimulating protein of p53) family, is an evolutionarily conserved inhibitor of p53 which is frequently upregulated in human cancers. However, little is known about the role of iASPP under physiological conditions. Here
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