C1427

Cytochrome b₅ human

recombinant, expressed in E. coli (histidine-tagged), ≥90% (SDS-PAGE)

• Sinônimo(s): Cytochrome b5
• Número MDL: MFCD02687283
• Código UNSPSC: 12161501
• NACRES: NA.47

Propriedades

• fonte biológica: human
• Nível de qualidade: 200
• recombinante: expressed in E. coli (histidine-tagged)
• Ensaio: ≥90% (SDS-PAGE)
• forma: solution
• peso molecular: 16 kDa
• técnica(s): inhibition assay: suitable
• adequação: suitable for molecular biology
• nº de adesão UniProt: P00167
• Condições de expedição: dry ice
• temperatura de armazenamento: −70°C
• Informações sobre genes: human ... CYB5A(1528)

Descrição

Descrição geral

Cytochrome b5 (CYB5A) gene codes for cytochrome b5 protein. It is located on human chromosome 18. It is co-expressed in the Leydig cells of the testis and theca cells of the ovary.

Ações bioquímicas/fisiológicas

Cytochrome b5 (CYB5A) mediates NADH-dependent reduction of methemoglobin (erythrocytes), electron transfer to fatty-acid desaturases (mainly liver) and steroid and xenobiotics metabolism by cytochrome P450 enzymes. It is essential for androstenedione synthesis. CYB5A helps in the efficient synthesis of gonadal androgen.

Cytochrome b₅ is a membrane-bound heme-containing protein that enhances the catalytic efficiency of some P450 isoforms. Its mechanism of action is unknown.

forma física

Solution in 20 mM phosphate buffer, pH 7.25, with 0.1 mM EDTA

Nota de análise

Heme content measured using difference spectroscopy. An extinction coefficient of 100 mM^-1 for the absorbance difference between 490 nm and 424 nm is used to calculate the concentration of cytochrome b₅.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)