A2668
Anti-Actin antibody produced in rabbit
whole antiserum
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About This Item
Número MDL:
Código UNSPSC:
12352203
Produtos recomendados
fonte biológica
rabbit
conjugado
unconjugated
forma do anticorpo
whole antiserum
tipo de produto de anticorpo
primary antibodies
clone
polyclonal
contém
15 mM sodium azide
reatividade de espécies
mouse, rat, human, chicken
técnica(s)
indirect immunofluorescence: 1:40 using cultured chicken fibroblasts
western blot: suitable using rabbit skeletal muscle α-actin1
nº de adesão UniProt
Condições de expedição
dry ice
temperatura de armazenamento
−20°C
Informações sobre genes
human ... ACTA1(58)
mouse ... Acta1(11459)
rat ... Acta1(29437)
Descrição geral
Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in all cell types. It varies in amount, being related to the type of differentiation and to the func tional state of cells and tissues. Actin can be found in two different forms of aggregation, the globular or the fibrillar state, and at least six distinct isoforms occur in vertebrates. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three α actins (skeletal, cardiac, smooth), one β actin (β-non-muscle) and two γ actins (γ smooth muscle and γ non-muscle). Difficulties have been encountered in producing polyclonal antibodies due to the highly conserved nature of actin.
Rabbit Anti-Actin reacts with pure actin by counter immunoelectrophoresis. The product also stains the I-bands of stretched skeletal muscle.
Rabbit polyclonal anti-Actin antibody reacts with rat, chicken, human, and mouse actin. By immunoblotting, cross-reactivity with other proteins may be observed.
Imunogênio
actin from chicken back muscle
Aplicação
May be used for studying the intracellular distribution of actin structures and their functions in the contractile system.
Rabbit anti-actin antibody has been used for western blot assays using mouse lung homogenates and human umbilical artery endothelial cell (HUAEC) lysates.
Rabbit anti-actin antibody was used to visualize actin in human sperm smears. Samples were incubated with the antibody (1:80) for 2 hours at 37 degrees.
Fluorescent microscopy was performed on bovine fallopian tube epithilial cells to visualize actin aggregates following inoculation with c. parvum sporozites using the rabbit anti-actin antibody from Sigma.
Fluorescent microscopy was performed on bovine fallopian tube epithilial cells to visualize actin aggregates following inoculation with c. parvum sporozites using the rabbit anti-actin antibody from Sigma.
Rabbit polyclonal anti-Actin antibody is used to tag actin for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of actin in stress fibers and I-bands of skeletal muscle cells.
forma física
Rabbit Anti-Actin is supplied as a liquid containing 0.1% sodium azide as preservative.
Outras notas
To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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