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90819

Sigma-Aldrich

Bis(2,2′-bipyridine)-(5-aminophenanthroline)ruthenium bis(hexafluorophosphate)

BioReagent, suitable for fluorescence

Sinônimo(s):

Ru(bpy)2(phen-5-NH2)(PF6)2

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About This Item

Fórmula empírica (Notação de Hill):
C32H25N7Ru · 2 PF6
Número CAS:
Peso molecular:
898.59
Número MDL:
Código UNSPSC:
12171500
ID de substância PubChem:
NACRES:
NA.21

linha de produto

BioReagent

Nível de qualidade

forma

powder

adequação da reação

core: ruthenium
reaction type: Photocatalysis
reagent type: catalyst

fluorescência

λex 458 nm; λem 612 nm in 0.1 M Tris pH 7.0

adequação

suitable for fluorescence

aplicação(ões)

diagnostic assay manufacturing
hematology
histology

temperatura de armazenamento

−20°C

cadeia de caracteres SMILES

[Ru++].F[P-](F)(F)(F)(F)F.F[P-](F)(F)(F)(F)F.c1ccc(nc1)-c2ccccn2.c3ccc(nc3)-c4ccccn4.Nc5cc6cccnc6c7ncccc57

InChI

1S/C12H9N3.2C10H8N2.2F6P.Ru/c13-10-7-8-3-1-5-14-11(8)12-9(10)4-2-6-15-12;2*1-3-7-11-9(5-1)10-6-2-4-8-12-10;2*1-7(2,3,4,5)6;/h1-7H,13H2;2*1-8H;;;/q;;;2*-1;+2

chave InChI

QQMMCXKCYXQNNA-UHFFFAOYSA-N

Aplicação

Used for photoinduced intramolecular electron transfer in peptide-bridged molecules.

Características e benefícios

Sigma-Aldrich offers a series of reactive dyes based on ruthenium complexes for use in fluorescence polarization assays and fluorescence lifetime imaging applications. They have been designed specifically to have long fluorescence lifetimes. These dyes were first synthesized in the labs of Dr. Joseph Lakowicz at the Center for Fluorescence Microscopy. These laser-excitable metal ligand complexes have fluorescence lifetimes near 500 nanoseconds.
These dyes can be used to label amines on biomolecules under mild conditions. Advantages of these dyes as protein labels include high photostability, good water solubility, a lack of dye-dye interactions and large Stokes′ shifts.
In addition, the fluorescence signal of long-lived fluorophores can be gated to eliminate the emission from short-lifetime fluorophores and autofluorescence from cells and biomolecules to further improve sensitivity.

Embalagem

Bottomless glass bottle. Contents are inside inserted fused cone.

Outras notas

Photoinduced intramolecular electron transfer in peptide-bridged molecules

produto relacionado

Nº do produto
Descrição
Preços

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


Certificados de análise (COA)

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K.S. Schanze et al.
Journal of the American Chemical Society, 110, 1180-1180 (1988)
E Terpetschnig et al.
Analytical biochemistry, 251(2), 241-245 (1997-09-23)
We report the synthesis and spectral properties of a long-lifetime luminescent Ru complex containing a sulfhydryl-reactive maleimide group, [Ru (2,2'-bipyridine)2(1, 10-phenanthroline-5-maleimide)](PF6)2. [Ru(bpy)2(phen-mi)]2+ was covalently linked to human serum albumin, immunoglobulin G, and beta-galactosidase. The lifetimes for probe bound to proteins
Tao Wang et al.
Analytica chimica acta, 1062, 124-130 (2019-04-06)
Vibrio parahaemolyticus (VP), as a kind of harmful bacterium to human beings, its rapid and accurate detection is particularly important. Herein, we developed a simple and efficient dual-modal Faraday cage-type immunosensor for ultrasensitive detection of VP based on electrochemiluminescence (ECL)

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