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83702

Sigma-Aldrich

Rhodamine 123

BioReagent, for fluorescence, ≥85% (HPLC)

Sinônimo(s):

2-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic acid methyl ester

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About This Item

Fórmula linear:
C21H17ClN2O3
Número CAS:
62669-70-9
Peso molecular:
380.82
Beilstein:
6030951
Número CE:
263-687-8
Número MDL:
MFCD00012664
Código UNSPSC:
12352116
ID de substância PubChem:
57652966
NACRES:
NA.32

grau

for fluorescence

linha de produto

BioReagent

Ensaio

≥85% (HPLC)

forma

powder

Impurezas

≤10% water

solubilidade

ethanol: 20 mg/mL, clear, red

adequação

in accordance for fluorescence

cadeia de caracteres SMILES

Cl[H].COC(=O)c1ccccc1C2=C3C=CC(=N)C=C3Oc4cc(N)ccc24

InChI

1S/C21H16N2O3.ClH/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20;/h2-11,22H,23H2,1H3;1H

chave InChI

MYFATKRONKHHQL-UHFFFAOYSA-N

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Descrição geral

Rhodamine 123 is a fluorescent dye.

Aplicação

Rhodamine 123 has been used:
  • as a substrate for P-glycoprotein (P-gp) to read out functional transporter activity in fluorescent accumulation assays
  • to label the cells for estimating the P-gp expression by flow cytometry
  • in transport studies, was added to the apical to basolateral (AB-transport) or basolateral to apical (BA-transport)
  • the detection of mitochondrial membrane potential (ΔΨm)

Ações bioquímicas/fisiológicas

Rhodamine 123 is usually used as a functional reporter for P-glycoprotein (P-gp) in flow cytometry.
Fluorescent dye most commonly used in flow cytometry as functional reporter for Pgp. Functional assays for MDR proteins are better prognostic indicators in cancer therapy than levels of MDR protein expression. Rh 123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA (7-amino-4-methylcoumarin-3-acetic acid). Recent reports indicate Rh 123 may also be a substrate of MRP1.

Outras notas

Supravital mitochondrial-specific dye

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Differentiation and characterization of human pluripotent stem cell-derived brain microvascular endothelial cells
Stebbins MJ, et al.
Methods, 101, 93-102 (2016)
Assessment of the chemotherapeutic potential of a new camptothecin derivative, ZBH-1205
Wu D, et al.
Archives of Biochemistry and Biophysics, 604, 74-85 (2016)
Evidence of apoptosis in Raillietina echinobothrida induced by methanolic extracts of three traditional medicinal plants of Northeast India
Giri BR, et al.
Experimental Parasitology, 134(4), 466-473 (2013)
L Benel et al.
Basic and applied histochemistry, 33(2), 71-80 (1989-01-01)
The use of the supravital mitochondrial-specific dye Rhodamine 123 (Rh 123) in combination with flow cytometry permits the monitoring of the changes in the mitochondrial transmembrane potential, reflecting the overall mitochondrial activity of the living cell. While this probe appears
X Ronot et al.
Biology of the cell, 57(1), 1-7 (1986-01-01)
Flow cytometry combines the advantages of microscopy and biochemical analysis in a single highly sensitive technique for a rapid examination of numerous individual living cells. It has become a potent and essential tool in the studies of the physiology of
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