M0428
Middlebrook 7H11 Agar Base
suitable for microbiology, NutriSelect® Plus
Sinônimo(s):
Media for culturing Mycobacteria
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About This Item
Código UNSPSC:
41171609
NACRES:
NA.85
Produtos recomendados
esterilidade
non-sterile
Nível de qualidade
linha de produto
BioChemika
forma
powder
prazo de validade
limited shelf life, expiry date on the label
fabricante/nome comercial
NutriSelect® Plus
condição de armazenamento
protect from light at room temperature
técnica(s)
microbe id | susceptibility testing: suitable
microbiological culture: suitable
pH final
6.6±0.2 (25 °C)
aplicação(ões)
clinical testing
food and beverages
life science and biopharma
veterinary
microbiology
adequação
mycobacteria
Categorias relacionadas
Descrição geral
Middlebrook 7H11 Agar Base media is used for the isolation, cultivation, and sensitivity testing of Mycobacterium species. This agar base contains many inorganic salts which help the growth of Mycobacteria. Citric acid formed from sodium citrate helps in retaining inorganic cations in solution. Glycerol supplies carbon and energy. The Middlebrook OADC Supplement (M0678) contains oleic acid, bovine albumin, sodium chloride, dextrose and catalase. Oleic acid and other long chain fatty acids are essential for the metabolism of Mycobacteria. Dextrose is an energy source. Catalase neutralizes toxic peroxides. Albumin protects tubercle bacilli from toxic agents. Malachite green partially inhibits other bacteria.
Aplicação
There are two types of solid culture media for the primary isolation of Mycobacteria. Egg-based (Lowenstein’s formulation) and agar-based (Middlebrook formulation)
The latter has the following advantages over the egg-based media
The Middlebrook 7H11 agar base does not liquify easily in the presence of contaminating proteolytic organisms
There is less inactivation of added drugs when egg ingredients are absent
The latter has the following advantages over the egg-based media
The Middlebrook 7H11 agar base does not liquify easily in the presence of contaminating proteolytic organisms
There is less inactivation of added drugs when egg ingredients are absent
Componentes
Ingredients (g/L)
Casein enzymatic hydrolysate, 1.00
Ammonium sulfate, 0.50
Monopotassium phosphate, 1.50
Disodium phosphate, 1.50
Sodium citrate, 0.40
Magnesium sulfate, 0.05
L-Glutamic acid, 0.50
Ferric ammonium citrate, 0.04
Pyridoxine, 0.001
Biotin, 0.0005
Malachite green, 0.001
Agar, 15.00
Casein enzymatic hydrolysate, 1.00
Ammonium sulfate, 0.50
Monopotassium phosphate, 1.50
Disodium phosphate, 1.50
Sodium citrate, 0.40
Magnesium sulfate, 0.05
L-Glutamic acid, 0.50
Ferric ammonium citrate, 0.04
Pyridoxine, 0.001
Biotin, 0.0005
Malachite green, 0.001
Agar, 15.00
Nota de preparo
Suspend 10.25 g of Middlebrook 7H11 Agar Base in 450 ml of distilled water. Add 2.5 ml of glycerol (Cat. No. 49767). Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool to 50°C and aseptically add 1 vial Middlebrook OADC Growth Supplement (M0678). Mix thoroughly before dispensing.
Nota de rodapé
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Informações legais
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
produto relacionado
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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Artigos
Identifying Mycobacterium species crucial due to varying clinical significance; some pathogenic, others not.
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